Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22920 | 3' | -54.3 | NC_005137.2 | + | 25857 | 0.77 | 0.372143 |
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Target: 5'- gACU-CGCCGACAcCGCGUcccAAUCCACCUu -3' miRNA: 3'- -UGAuGCGGCUGU-GCGUA---UUGGGUGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 20954 | 0.68 | 0.839058 |
|
Target: 5'- gACUGCGUcugcugccugCGGCGCGCGUuuuGCCgCGCUUg -3' miRNA: 3'- -UGAUGCG----------GCUGUGCGUAu--UGG-GUGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 16540 | 1.07 | 0.0046 |
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Target: 5'- gACUACGCCGACACGCAUAACCCACCUc -3' miRNA: 3'- -UGAUGCGGCUGUGCGUAUUGGGUGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 13263 | 0.7 | 0.766475 |
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Target: 5'- gGCUAUagGCCGGCGUGgGUAGCaCCGCCa -3' miRNA: 3'- -UGAUG--CGGCUGUGCgUAUUG-GGUGGa -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 7847 | 0.66 | 0.93922 |
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Target: 5'- -aUGCGCCG-CAgGCAUuacguGCCCAUg- -3' miRNA: 3'- ugAUGCGGCuGUgCGUAu----UGGGUGga -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 5273 | 0.66 | 0.92355 |
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Target: 5'- ---uUGUCGGCGCGCGUcaucaaaagaaAGCUCGCCUc -3' miRNA: 3'- ugauGCGGCUGUGCGUA-----------UUGGGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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