Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22920 | 3' | -54.3 | NC_005137.2 | + | 110598 | 0.67 | 0.89245 |
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Target: 5'- --cGCGCCGGC-CGCGgugugcacGCUCACCg -3' miRNA: 3'- ugaUGCGGCUGuGCGUau------UGGGUGGa -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 28033 | 0.67 | 0.8855 |
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Target: 5'- --gGCGCCGcCACGCGaucgccAACCCGCa- -3' miRNA: 3'- ugaUGCGGCuGUGCGUa-----UUGGGUGga -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 93620 | 0.68 | 0.870901 |
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Target: 5'- aACgGCGCUGGCGCGCAccguGCCCGu-- -3' miRNA: 3'- -UGaUGCGGCUGUGCGUau--UGGGUgga -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 130772 | 0.68 | 0.855403 |
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Target: 5'- gGCUuuGUCGGCGCGCGUGuCCgCGCUUu -3' miRNA: 3'- -UGAugCGGCUGUGCGUAUuGG-GUGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 61413 | 0.68 | 0.855403 |
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Target: 5'- aACUgACGCCGACAauUAcUAAUCCGCCg -3' miRNA: 3'- -UGA-UGCGGCUGUgcGU-AUUGGGUGGa -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 7847 | 0.66 | 0.93922 |
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Target: 5'- -aUGCGCCG-CAgGCAUuacguGCCCAUg- -3' miRNA: 3'- ugAUGCGGCuGUgCGUAu----UGGGUGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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