Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22920 | 3' | -54.3 | NC_005137.2 | + | 103346 | 0.67 | 0.911851 |
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Target: 5'- uACUcCGUuuuCGACG-GCGUAGCCCACUa -3' miRNA: 3'- -UGAuGCG---GCUGUgCGUAUUGGGUGGa -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 33939 | 0.67 | 0.911851 |
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Target: 5'- uGCaACGuuGGCACaGCAcAACgCCGCCg -3' miRNA: 3'- -UGaUGCggCUGUG-CGUaUUG-GGUGGa -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 106432 | 0.66 | 0.917825 |
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Target: 5'- --gGCGCCGugGgGCAUcGCgUGCCUg -3' miRNA: 3'- ugaUGCGGCugUgCGUAuUGgGUGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 5273 | 0.66 | 0.92355 |
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Target: 5'- ---uUGUCGGCGCGCGUcaucaaaagaaAGCUCGCCUc -3' miRNA: 3'- ugauGCGGCUGUGCGUA-----------UUGGGUGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 33351 | 0.66 | 0.934247 |
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Target: 5'- -gUGCGCucuuCGACGCGCAauUGACCCGu-- -3' miRNA: 3'- ugAUGCG----GCUGUGCGU--AUUGGGUgga -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 7847 | 0.66 | 0.93922 |
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Target: 5'- -aUGCGCCG-CAgGCAUuacguGCCCAUg- -3' miRNA: 3'- ugAUGCGGCuGUgCGUAu----UGGGUGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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