Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22957 | 5' | -49.1 | NC_005137.2 | + | 61042 | 0.66 | 0.9981 |
Target: 5'- cGGCGGu--CUUGuaaucguACGCGUUugcGCUGCa -3' miRNA: 3'- cCCGUCuuuGAACu------UGUGCAA---CGGCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 7679 | 0.66 | 0.9981 |
Target: 5'- aGGCAac-GCUuccaUGGGCACGUaaUGCCuGCg -3' miRNA: 3'- cCCGUcuuUGA----ACUUGUGCA--ACGG-CG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 52063 | 0.66 | 0.9981 |
Target: 5'- -cGCAuGAcGCga-AACGCGUUGUCGCa -3' miRNA: 3'- ccCGU-CUuUGaacUUGUGCAACGGCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 58863 | 0.66 | 0.9981 |
Target: 5'- --cCAGAAAacUGuGCACGgUGCCGCg -3' miRNA: 3'- cccGUCUUUgaACuUGUGCaACGGCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 110620 | 0.66 | 0.9981 |
Target: 5'- uGGCGGuuaguGACcccgUGuACGCGccgGCCGCg -3' miRNA: 3'- cCCGUCu----UUGa---ACuUGUGCaa-CGGCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 56225 | 0.66 | 0.997722 |
Target: 5'- cGGUAGugguuGCguuuUGGGgACGUgaUGCCGCa -3' miRNA: 3'- cCCGUCuu---UGa---ACUUgUGCA--ACGGCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 42410 | 0.66 | 0.996777 |
Target: 5'- cGGCGGugccccGCcgGGACACGUnauguguccGCCGCg -3' miRNA: 3'- cCCGUCuu----UGaaCUUGUGCAa--------CGGCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 118132 | 0.66 | 0.996777 |
Target: 5'- uGGCGGGugAGCUgcUGuuguuGC-CGUUGCUGCu -3' miRNA: 3'- cCCGUCU--UUGA--ACu----UGuGCAACGGCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 68776 | 0.67 | 0.99553 |
Target: 5'- uGGCGcGAAAaccgUGAAacUACGgUGCCGCg -3' miRNA: 3'- cCCGU-CUUUga--ACUU--GUGCaACGGCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 37398 | 0.67 | 0.994773 |
Target: 5'- --aCGGAAugUUGAAUAUGggguagcGCCGCg -3' miRNA: 3'- cccGUCUUugAACUUGUGCaa-----CGGCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 5453 | 0.67 | 0.993915 |
Target: 5'- uGGGCGGAAACUcGAGCGaGUcuagCGCa -3' miRNA: 3'- -CCCGUCUUUGAaCUUGUgCAacg-GCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 85452 | 0.67 | 0.993915 |
Target: 5'- cGGCAcGA----UGAACACGguUUGCCGUu -3' miRNA: 3'- cCCGU-CUuugaACUUGUGC--AACGGCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 24631 | 0.67 | 0.992948 |
Target: 5'- cGGGaaaaAAACUUGAuCACG--GCCGCa -3' miRNA: 3'- -CCCguc-UUUGAACUuGUGCaaCGGCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 119569 | 0.67 | 0.992948 |
Target: 5'- cGGUAGAgcGACgcacgcUGAGCACG-UGCCa- -3' miRNA: 3'- cCCGUCU--UUGa-----ACUUGUGCaACGGcg -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 24387 | 0.67 | 0.992948 |
Target: 5'- uGGGUuu--GCUguUGGACGCGgcgucagGCCGCc -3' miRNA: 3'- -CCCGucuuUGA--ACUUGUGCaa-----CGGCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 63182 | 0.68 | 0.989293 |
Target: 5'- uGGaauaauaAGAAGCUUGccGACACGccggcGCCGCa -3' miRNA: 3'- cCCg------UCUUUGAAC--UUGUGCaa---CGGCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 59759 | 0.68 | 0.987311 |
Target: 5'- aGGGCguagaaguguuuuuAGAGAUUUGcAACACGguggggUUGCgCGCg -3' miRNA: 3'- -CCCG--------------UCUUUGAAC-UUGUGC------AACG-GCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 76535 | 0.68 | 0.986132 |
Target: 5'- cGGCAGuuuGACgcuaaUUGAGCAUuUUGCCGg -3' miRNA: 3'- cCCGUCu--UUG-----AACUUGUGcAACGGCg -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 52602 | 0.68 | 0.986132 |
Target: 5'- cGGCGGAGgcGCUUGAAacggaaGCGc-GCCGUu -3' miRNA: 3'- cCCGUCUU--UGAACUUg-----UGCaaCGGCG- -5' |
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22957 | 5' | -49.1 | NC_005137.2 | + | 7315 | 0.68 | 0.985602 |
Target: 5'- cGGGCAGGAugcggucaaaguGCUggcgccccgccaguUGAGuCAUGUUGCUGg -3' miRNA: 3'- -CCCGUCUU------------UGA--------------ACUU-GUGCAACGGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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