Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22964 | 5' | -59.1 | NC_005137.2 | + | 96566 | 0.68 | 0.660901 |
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Target: 5'- gGCgCGUCGcCGCGCGGGuagcGGUCG-GCGc -3' miRNA: 3'- -CGgGCGGCuGCGCGUUC----UCAGCaCGC- -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 100145 | 0.67 | 0.710421 |
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Target: 5'- aGCUCGCCcacguacgGGCGCGCc-GGGUCGUcaGCu -3' miRNA: 3'- -CGGGCGG--------CUGCGCGuuCUCAGCA--CGc -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 100185 | 0.66 | 0.785881 |
|
Target: 5'- cGCCgCGUCGGaccgcgugcuUGCGCAccgcuGGAGUUGgugGCGg -3' miRNA: 3'- -CGG-GCGGCU----------GCGCGU-----UCUCAGCa--CGC- -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 104052 | 0.72 | 0.412854 |
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Target: 5'- cGCCCuuugugGCCGugGCGCcuucGUCGUGCu -3' miRNA: 3'- -CGGG------CGGCugCGCGuucuCAGCACGc -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 110554 | 0.67 | 0.710421 |
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Target: 5'- uGCCCGCCGAUGUGCGcccgcaccuGcGUCuugGCGc -3' miRNA: 3'- -CGGGCGGCUGCGCGUu--------CuCAGca-CGC- -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 110823 | 1.1 | 0.00129 |
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Target: 5'- cGCCCGCCGACGCGCAAGAGUCGUGCGc -3' miRNA: 3'- -CGGGCGGCUGCGCGUUCUCAGCACGC- -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 117912 | 0.69 | 0.590841 |
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Target: 5'- gGCCCGUCGACGauaaGCAGcucGAGaagCGUGUu -3' miRNA: 3'- -CGGGCGGCUGCg---CGUU---CUCa--GCACGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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