miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22964 5' -59.1 NC_005137.2 + 63774 0.66 0.748938
Target:  5'- uGCCCGuaGAacCGUGCGAaGGUCGcGCGc -3'
miRNA:   3'- -CGGGCggCU--GCGCGUUcUCAGCaCGC- -5'
22964 5' -59.1 NC_005137.2 + 81594 0.66 0.758343
Target:  5'- --aCGCgUGACGCgGCAgcaguaagcAGGGUCGUGCa -3'
miRNA:   3'- cggGCG-GCUGCG-CGU---------UCUCAGCACGc -5'
22964 5' -59.1 NC_005137.2 + 63666 0.66 0.767641
Target:  5'- cGCCCGCggucaccgaaccCGACGCGUuuGuGUUGacggGCGa -3'
miRNA:   3'- -CGGGCG------------GCUGCGCGuuCuCAGCa---CGC- -5'
22964 5' -59.1 NC_005137.2 + 61485 0.66 0.767641
Target:  5'- uGUCCGCgGACGCGgAc-AGUgGUGCc -3'
miRNA:   3'- -CGGGCGgCUGCGCgUucUCAgCACGc -5'
22964 5' -59.1 NC_005137.2 + 29837 0.66 0.785881
Target:  5'- uUCCGCCG-CGCuaaaaAAGGGUCGcGCGc -3'
miRNA:   3'- cGGGCGGCuGCGcg---UUCUCAGCaCGC- -5'
22964 5' -59.1 NC_005137.2 + 66319 0.66 0.785881
Target:  5'- cGCCCcaGCCGAUGCGCGuaccaauuGUgGUcGCGu -3'
miRNA:   3'- -CGGG--CGGCUGCGCGUucu-----CAgCA-CGC- -5'
22964 5' -59.1 NC_005137.2 + 100185 0.66 0.785881
Target:  5'- cGCCgCGUCGGaccgcgugcuUGCGCAccgcuGGAGUUGgugGCGg -3'
miRNA:   3'- -CGG-GCGGCU----------GCGCGU-----UCUCAGCa--CGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.