Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22964 | 5' | -59.1 | NC_005137.2 | + | 63774 | 0.66 | 0.748938 |
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Target: 5'- uGCCCGuaGAacCGUGCGAaGGUCGcGCGc -3' miRNA: 3'- -CGGGCggCU--GCGCGUUcUCAGCaCGC- -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 81594 | 0.66 | 0.758343 |
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Target: 5'- --aCGCgUGACGCgGCAgcaguaagcAGGGUCGUGCa -3' miRNA: 3'- cggGCG-GCUGCG-CGU---------UCUCAGCACGc -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 61485 | 0.66 | 0.767641 |
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Target: 5'- uGUCCGCgGACGCGgAc-AGUgGUGCc -3' miRNA: 3'- -CGGGCGgCUGCGCgUucUCAgCACGc -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 63666 | 0.66 | 0.767641 |
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Target: 5'- cGCCCGCggucaccgaaccCGACGCGUuuGuGUUGacggGCGa -3' miRNA: 3'- -CGGGCG------------GCUGCGCGuuCuCAGCa---CGC- -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 100185 | 0.66 | 0.785881 |
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Target: 5'- cGCCgCGUCGGaccgcgugcuUGCGCAccgcuGGAGUUGgugGCGg -3' miRNA: 3'- -CGG-GCGGCU----------GCGCGU-----UCUCAGCa--CGC- -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 29837 | 0.66 | 0.785881 |
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Target: 5'- uUCCGCCG-CGCuaaaaAAGGGUCGcGCGc -3' miRNA: 3'- cGGGCGGCuGCGcg---UUCUCAGCaCGC- -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 66319 | 0.66 | 0.785881 |
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Target: 5'- cGCCCcaGCCGAUGCGCGuaccaauuGUgGUcGCGu -3' miRNA: 3'- -CGGG--CGGCUGCGCGUucu-----CAgCA-CGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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