Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22964 | 5' | -59.1 | NC_005137.2 | + | 117912 | 0.69 | 0.590841 |
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Target: 5'- gGCCCGUCGACGauaaGCAGcucGAGaagCGUGUu -3' miRNA: 3'- -CGGGCGGCUGCg---CGUU---CUCa--GCACGc -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 22513 | 0.7 | 0.516391 |
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Target: 5'- cGCCUGCCGACGUGCAcccauuGcuugugucgccaaauUCGUGCa -3' miRNA: 3'- -CGGGCGGCUGCGCGUucu---C---------------AGCACGc -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 30797 | 0.7 | 0.509707 |
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Target: 5'- -gUCGUCGACGCGCAccagcaacacguugGGAGU-GUGCGc -3' miRNA: 3'- cgGGCGGCUGCGCGU--------------UCUCAgCACGC- -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 104052 | 0.72 | 0.412854 |
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Target: 5'- cGCCCuuugugGCCGugGCGCcuucGUCGUGCu -3' miRNA: 3'- -CGGG------CGGCugCGCGuucuCAGCACGc -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 96306 | 0.73 | 0.387792 |
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Target: 5'- cGCCgacaCGCCGGCGCGCAAGuGUgGacUGCc -3' miRNA: 3'- -CGG----GCGGCUGCGCGUUCuCAgC--ACGc -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 33048 | 0.73 | 0.355969 |
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Target: 5'- aGCaaaUGCCGACGCGUucuAGAGaUUGUGCa -3' miRNA: 3'- -CGg--GCGGCUGCGCGu--UCUC-AGCACGc -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 110823 | 1.1 | 0.00129 |
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Target: 5'- cGCCCGCCGACGCGCAAGAGUCGUGCGc -3' miRNA: 3'- -CGGGCGGCUGCGCGUUCUCAGCACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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