Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22964 | 5' | -59.1 | NC_005137.2 | + | 67705 | 0.67 | 0.710421 |
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Target: 5'- uCCCGCuCGGCGCGCGu---UUGUGCc -3' miRNA: 3'- cGGGCG-GCUGCGCGUucucAGCACGc -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 70125 | 0.67 | 0.739435 |
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Target: 5'- uCCCGCUGugGUc--GGuGUCGUGCa -3' miRNA: 3'- cGGGCGGCugCGcguUCuCAGCACGc -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 95637 | 0.67 | 0.739435 |
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Target: 5'- cGCCCGUgCGGCGCGCAuccgaccGGUaCGUuGCa -3' miRNA: 3'- -CGGGCG-GCUGCGCGUuc-----UCA-GCA-CGc -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 61392 | 0.66 | 0.746097 |
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Target: 5'- aUCCGCCGcguuuGCGCGCAuuauGAGUUuguacaagaacaaaGUGUGg -3' miRNA: 3'- cGGGCGGC-----UGCGCGUu---CUCAG--------------CACGC- -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 100185 | 0.66 | 0.785881 |
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Target: 5'- cGCCgCGUCGGaccgcgugcuUGCGCAccgcuGGAGUUGgugGCGg -3' miRNA: 3'- -CGG-GCGGCU----------GCGCGU-----UCUCAGCa--CGC- -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 66319 | 0.66 | 0.785881 |
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Target: 5'- cGCCCcaGCCGAUGCGCGuaccaauuGUgGUcGCGu -3' miRNA: 3'- -CGGG--CGGCUGCGCGUucu-----CAgCA-CGC- -5' |
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| 22964 | 5' | -59.1 | NC_005137.2 | + | 22513 | 0.7 | 0.516391 |
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Target: 5'- cGCCUGCCGACGUGCAcccauuGcuugugucgccaaauUCGUGCa -3' miRNA: 3'- -CGGGCGGCUGCGCGUucu---C---------------AGCACGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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