Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22972 | 5' | -52.2 | NC_005137.2 | + | 123050 | 0.69 | 0.885522 |
Target: 5'- aCGGGCcgccggUCGAGguCGCGGCGCGCa--- -3' miRNA: 3'- -GCUCG------AGUUUguGCGCCGUGUGaaca -5' |
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22972 | 5' | -52.2 | NC_005137.2 | + | 125277 | 0.7 | 0.854867 |
Target: 5'- aGAcGUUCGAGCgGCGCGGCccgcCGCUUGa -3' miRNA: 3'- gCU-CGAGUUUG-UGCGCCGu---GUGAACa -5' |
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22972 | 5' | -52.2 | NC_005137.2 | + | 126058 | 0.66 | 0.9767 |
Target: 5'- -uGGCUgGAGCugGCuGGCugGCUg-- -3' miRNA: 3'- gcUCGAgUUUGugCG-CCGugUGAaca -5' |
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22972 | 5' | -52.2 | NC_005137.2 | + | 129526 | 0.66 | 0.9767 |
Target: 5'- aCGuGCUggCGAGCAuUGUGGCGCGCaUUGa -3' miRNA: 3'- -GCuCGA--GUUUGU-GCGCCGUGUG-AACa -5' |
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22972 | 5' | -52.2 | NC_005137.2 | + | 130650 | 1.09 | 0.005677 |
Target: 5'- gCGAGCUCAAACACGCGGCACACUUGUa -3' miRNA: 3'- -GCUCGAGUUUGUGCGCCGUGUGAACA- -5' |
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22972 | 5' | -52.2 | NC_005137.2 | + | 130788 | 0.67 | 0.949272 |
Target: 5'- uGAGCUCGcuuuuGCugGCuuugucGGCGCGCgUGUc -3' miRNA: 3'- gCUCGAGUu----UGugCG------CCGUGUGaACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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