Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23027 | 3' | -59.6 | NC_005178.1 | + | 15092 | 0.73 | 0.127334 |
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Target: 5'- gCCCCACa-CCAGGCAGUGAuaGGCccgcugcauGGCg -3' miRNA: 3'- gGGGGUGacGGUCCGUUACU--CCG---------CCG- -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 9585 | 0.73 | 0.134634 |
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Target: 5'- uUCCCGC-GCCAGGCGGcgcuGGCGGUc -3' miRNA: 3'- gGGGGUGaCGGUCCGUUacu-CCGCCG- -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 36388 | 0.69 | 0.22534 |
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Target: 5'- uCgUCCGCUGUCGGGCAucagauUGAGaaaCGGCa -3' miRNA: 3'- -GgGGGUGACGGUCCGUu-----ACUCc--GCCG- -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 27935 | 0.69 | 0.250087 |
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Target: 5'- -aCCCAgUUGCCcuGGGCGGccugGAGGuCGGCg -3' miRNA: 3'- ggGGGU-GACGG--UCCGUUa---CUCC-GCCG- -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 11818 | 0.68 | 0.26328 |
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Target: 5'- aCCCCCAUgGCuCGGGCcAcgGccGUGGCa -3' miRNA: 3'- -GGGGGUGaCG-GUCCG-UuaCucCGCCG- -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 6888 | 1.14 | 0.000098 |
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Target: 5'- aCCCCCACUGCCAGGCAAUGAGGCGGCg -3' miRNA: 3'- -GGGGGUGACGGUCCGUUACUCCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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