Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23027 | 3' | -59.6 | NC_005178.1 | + | 2717 | 0.66 | 0.389327 |
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Target: 5'- gCCgCGCUGCUgaacgccgcccuGGGC---GAGGUGGCc -3' miRNA: 3'- gGGgGUGACGG------------UCCGuuaCUCCGCCG- -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 28086 | 0.66 | 0.364633 |
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Target: 5'- aCCCgCCAgcuaggggcgucuCUGCUuuccggguuuggccuGGGCGGUGGcaccgauGGCGGCg -3' miRNA: 3'- -GGG-GGU-------------GACGG---------------UCCGUUACU-------CCGCCG- -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 28494 | 0.67 | 0.345175 |
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Target: 5'- aCUCCGCUGCUgcuGGaCAucaaccaacgaggAUG-GGCGGCg -3' miRNA: 3'- gGGGGUGACGGu--CC-GU-------------UACuCCGCCG- -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 21769 | 0.67 | 0.306249 |
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Target: 5'- gCUCCGCUuuccaacgccaGCCAGGC-----GGCGGCg -3' miRNA: 3'- gGGGGUGA-----------CGGUCCGuuacuCCGCCG- -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 22261 | 0.68 | 0.297244 |
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Target: 5'- -gCCgACcGCCAGGCuggcccgccAGGCGGCg -3' miRNA: 3'- ggGGgUGaCGGUCCGuuac-----UCCGCCG- -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 16653 | 0.8 | 0.036916 |
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Target: 5'- aUCCCGCUGCCAGcCAAUggcGAGGCGGUc -3' miRNA: 3'- gGGGGUGACGGUCcGUUA---CUCCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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