Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23027 | 3' | -59.6 | NC_005178.1 | + | 6829 | 0.75 | 0.090728 |
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Target: 5'- gCCUCAUUGCCuGGCAGUGGGG-GuGCu -3' miRNA: 3'- gGGGGUGACGGuCCGUUACUCCgC-CG- -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 28328 | 0.75 | 0.088175 |
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Target: 5'- aCCCCACUGacaCCAuuggcGGCGGccagcGAGGCGGCa -3' miRNA: 3'- gGGGGUGAC---GGU-----CCGUUa----CUCCGCCG- -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 25495 | 0.76 | 0.080919 |
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Target: 5'- uUCCCGCUGUU--GCAggGAGGCGGCg -3' miRNA: 3'- gGGGGUGACGGucCGUuaCUCCGCCG- -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 7398 | 0.76 | 0.07863 |
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Target: 5'- gCCCCUcugucgGCU-CCAGGCGAUGAaGGCGGa -3' miRNA: 3'- -GGGGG------UGAcGGUCCGUUACU-CCGCCg -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 16653 | 0.8 | 0.036916 |
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Target: 5'- aUCCCGCUGCCAGcCAAUggcGAGGCGGUc -3' miRNA: 3'- gGGGGUGACGGUCcGUUA---CUCCGCCG- -5' |
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| 23027 | 3' | -59.6 | NC_005178.1 | + | 6888 | 1.14 | 0.000098 |
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Target: 5'- aCCCCCACUGCCAGGCAAUGAGGCGGCg -3' miRNA: 3'- -GGGGGUGACGGUCCGUUACUCCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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