miRNA: 3'- -UGGucaGCGGCG-GGuaCGCCGGUGGa -5'
miRNA: 3'- -UGG-UCAGCGGCGGg-UACGc-----CGGUGGa -5'
miRNA: 3'- -UGGUca-GCgGCGGguaCGCCGGUGGa -5'
miRNA: 3'- -UGGuCAGcgGCGGGUA---C-GCCGGUGGA- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23035 | 5' | -63 | NC_005178.1 | + | 9582 | 0.66 | 0.252562 |
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Target: 5'- cGCCg--CGCUGCgCUggGCGGUCGCCg -3' miRNA: 3'- -UGGucaGCGGCG-GGuaCGCCGGUGGa -5' |
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| 23035 | 5' | -63 | NC_005178.1 | + | 22708 | 0.66 | 0.259043 |
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Target: 5'- cGCCgGGUCGCUGCCgaGUGCcauagcGCCAUCg -3' miRNA: 3'- -UGG-UCAGCGGCGGg-UACGc-----CGGUGGa -5' |
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| 23035 | 5' | -63 | NC_005178.1 | + | 17665 | 0.66 | 0.259043 |
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Target: 5'- gACCAcggCGaCGCCa--GCGGCCGCCg -3' miRNA: 3'- -UGGUca-GCgGCGGguaCGCCGGUGGa -5' |
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| 23035 | 5' | -63 | NC_005178.1 | + | 17729 | 0.66 | 0.259043 |
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Target: 5'- cGCUuGUCuaCGCCCGUcucGaCGGUCACCUg -3' miRNA: 3'- -UGGuCAGcgGCGGGUA---C-GCCGGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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