Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23036 | 5' | -56.9 | NC_005178.1 | + | 28131 | 0.7 | 0.268219 |
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Target: 5'- -cUGGCGggucGCCAUccUGGCCAGGUCg -3' miRNA: 3'- uuACUGCaa--CGGUAccGCCGGUCCAG- -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 11883 | 0.7 | 0.268219 |
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Target: 5'- gAAUGAuCGcgGUCAccagGGCGGCCAGGg- -3' miRNA: 3'- -UUACU-GCaaCGGUa---CCGCCGGUCCag -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 26367 | 0.7 | 0.254333 |
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Target: 5'- gAGUGACGccGCCAUGGCGGaCAGu-- -3' miRNA: 3'- -UUACUGCaaCGGUACCGCCgGUCcag -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 27938 | 0.75 | 0.122724 |
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Target: 5'- -uUGACccaGUUGCCcugGGCGGCCuggAGGUCg -3' miRNA: 3'- uuACUG---CAACGGua-CCGCCGG---UCCAG- -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 16629 | 1.07 | 0.000407 |
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Target: 5'- uAAUGACGUUGCCAUGGCGGCCAGGUCg -3' miRNA: 3'- -UUACUGCAACGGUACCGCCGGUCCAG- -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 24110 | 0.66 | 0.461089 |
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Target: 5'- gGGUGAUGUUGaCgGUGGCGGUCuugccgauAGGg- -3' miRNA: 3'- -UUACUGCAAC-GgUACCGCCGG--------UCCag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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