Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23036 | 5' | -56.9 | NC_005178.1 | + | 28131 | 0.7 | 0.268219 |
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Target: 5'- -cUGGCGggucGCCAUccUGGCCAGGUCg -3' miRNA: 3'- uuACUGCaa--CGGUAccGCCGGUCCAG- -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 28373 | 0.7 | 0.261201 |
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Target: 5'- --cGGCuucGCgGgUGGCGGCCAGGUCc -3' miRNA: 3'- uuaCUGcaaCGgU-ACCGCCGGUCCAG- -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 30159 | 0.69 | 0.290186 |
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Target: 5'- cGUGGCGUcgGCUAUacgGGCGGCCAGu-- -3' miRNA: 3'- uUACUGCAa-CGGUA---CCGCCGGUCcag -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 31896 | 0.67 | 0.410999 |
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Target: 5'- --cGACGUcGCCGggaUGGCGcuGCC-GGUCa -3' miRNA: 3'- uuaCUGCAaCGGU---ACCGC--CGGuCCAG- -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 35111 | 0.66 | 0.450808 |
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Target: 5'- --cGA-GcUGCCGcUGGCGGCC-GGUCc -3' miRNA: 3'- uuaCUgCaACGGU-ACCGCCGGuCCAG- -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 36140 | 0.69 | 0.290186 |
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Target: 5'- aGGUGGCGaacgagGCCAcgccUGcUGGCCAGGUCa -3' miRNA: 3'- -UUACUGCaa----CGGU----ACcGCCGGUCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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