Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23036 | 5' | -56.9 | NC_005178.1 | + | 10235 | 0.69 | 0.321634 |
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Target: 5'- --aGACcugGCCAUGGCGGCuCcuGUCg -3' miRNA: 3'- uuaCUGcaaCGGUACCGCCG-GucCAG- -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 9591 | 0.66 | 0.450808 |
|
Target: 5'- -uUGGCGUUcccgcGCCA-GGCGGCgCuggcGGUCu -3' miRNA: 3'- uuACUGCAA-----CGGUaCCGCCG-Gu---CCAG- -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 5893 | 0.66 | 0.450808 |
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Target: 5'- -uUGAgGgcGUaAUGGCGGCCguugcGGGUCa -3' miRNA: 3'- uuACUgCaaCGgUACCGCCGG-----UCCAG- -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 5705 | 0.69 | 0.321634 |
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Target: 5'- gGAUGGC-UUGCCAUcGGCGuuCAGGUUc -3' miRNA: 3'- -UUACUGcAACGGUA-CCGCcgGUCCAG- -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 5009 | 0.68 | 0.350314 |
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Target: 5'- cGAUGaucaGCGggcgGCCGcUGGCGGCCAguuccucggcgaucuGGUCg -3' miRNA: 3'- -UUAC----UGCaa--CGGU-ACCGCCGGU---------------CCAG- -5' |
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| 23036 | 5' | -56.9 | NC_005178.1 | + | 2897 | 0.69 | 0.312739 |
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Target: 5'- -cUGAUGcUGgaCAUGGCgcgccucGGCCAGGUCg -3' miRNA: 3'- uuACUGCaACg-GUACCG-------CCGGUCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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