Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23045 | 3' | -62.4 | NC_005178.1 | + | 31371 | 0.7 | 0.150515 |
Target: 5'- cGCGGCgCCAGGcgcauagcccggCGCUGG-GUCaCGGCUg -3' miRNA: 3'- -CGCCG-GGUCCa-----------GCGGCCaUAG-GCCGA- -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 14287 | 0.71 | 0.132176 |
Target: 5'- -aGGUCCAGGUCuUCGGc-UCCGGCa -3' miRNA: 3'- cgCCGGGUCCAGcGGCCauAGGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 4028 | 0.71 | 0.128274 |
Target: 5'- aGCGGCCgGGccucGUCGUCGGUgacgcgcAUCgGGCUg -3' miRNA: 3'- -CGCCGGgUC----CAGCGGCCA-------UAGgCCGA- -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 5923 | 0.71 | 0.115282 |
Target: 5'- gGCGGCCgAGG--GCCGGUGUagCGGCc -3' miRNA: 3'- -CGCCGGgUCCagCGGCCAUAg-GCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 33532 | 0.72 | 0.112154 |
Target: 5'- aGUGGUCCAGGUCgGCaCGGUGUgCaGGUg -3' miRNA: 3'- -CGCCGGGUCCAG-CG-GCCAUAgG-CCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 32281 | 0.73 | 0.09606 |
Target: 5'- gGCGGCCCAGGgcggCGUcgauggccaaacgcuCGGUGU-CGGCg -3' miRNA: 3'- -CGCCGGGUCCa---GCG---------------GCCAUAgGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 25137 | 0.73 | 0.08738 |
Target: 5'- aGCGGCCgAgcuGGUCGCgcUGGUGUCCuGCUg -3' miRNA: 3'- -CGCCGGgU---CCAGCG--GCCAUAGGcCGA- -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 10294 | 0.74 | 0.078124 |
Target: 5'- cCGGCCau-GUCGCggaCGGUAUCCGGCa -3' miRNA: 3'- cGCCGGgucCAGCG---GCCAUAGGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 5880 | 0.75 | 0.064141 |
Target: 5'- gGCGGCCguugCGGGUCa--GGUAUCCGGCg -3' miRNA: 3'- -CGCCGG----GUCCAGcggCCAUAGGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 21092 | 0.77 | 0.040673 |
Target: 5'- cGCGGCCCAGGUaggucagGCCGGUGg-CGGUg -3' miRNA: 3'- -CGCCGGGUCCAg------CGGCCAUagGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 24640 | 0.97 | 0.001207 |
Target: 5'- aGC-GCCCAGGUCGCCGGUAUCCGGCUu -3' miRNA: 3'- -CGcCGGGUCCAGCGGCCAUAGGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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