Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23045 | 3' | -62.4 | NC_005178.1 | + | 22877 | 0.67 | 0.236405 |
Target: 5'- cGUGGCCCAGGcuaUCGCCG--AUgCGGaCg -3' miRNA: 3'- -CGCCGGGUCC---AGCGGCcaUAgGCC-Ga -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 20326 | 0.67 | 0.242491 |
Target: 5'- cGCGGCCCu-GUCGCUGGaagaaGGCa -3' miRNA: 3'- -CGCCGGGucCAGCGGCCauaggCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 30861 | 0.66 | 0.261532 |
Target: 5'- cCGGCCCAGGUCgagGCCGcGaccgcugCCaGGCg -3' miRNA: 3'- cGCCGGGUCCAG---CGGC-Caua----GG-CCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 9659 | 0.66 | 0.268145 |
Target: 5'- cGCGGCCgGuGGcuUCGCCGag--CCGGCc -3' miRNA: 3'- -CGCCGGgU-CC--AGCGGCcauaGGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 20464 | 0.66 | 0.273531 |
Target: 5'- uUGGCCagguuGGUCGCCuGGgcguccagggCCGGCUu -3' miRNA: 3'- cGCCGGgu---CCAGCGG-CCaua-------GGCCGA- -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 12816 | 0.66 | 0.274892 |
Target: 5'- cGCGGCgCAGGgcgUCGCgGGUuuccuUCgGGUg -3' miRNA: 3'- -CGCCGgGUCC---AGCGgCCAu----AGgCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 32081 | 0.66 | 0.281773 |
Target: 5'- -aGGgUCAGGUCGUCGGcGUgggUCGGCa -3' miRNA: 3'- cgCCgGGUCCAGCGGCCaUA---GGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 31448 | 0.66 | 0.295946 |
Target: 5'- aGCuGCuCCAGGaacCGCUGGcggCCGGCUc -3' miRNA: 3'- -CGcCG-GGUCCa--GCGGCCauaGGCCGA- -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 11762 | 0.66 | 0.303237 |
Target: 5'- cGUGGCCCgagccauggGGGUucCGCCGcaggCCGGCc -3' miRNA: 3'- -CGCCGGG---------UCCA--GCGGCcauaGGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 31651 | 0.66 | 0.303237 |
Target: 5'- gGCGGaCCAGGUCcagGCCGG----CGGCg -3' miRNA: 3'- -CGCCgGGUCCAG---CGGCCauagGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 11876 | 0.66 | 0.303237 |
Target: 5'- cGCGGUcaCCAGGgcgGCCagGGUGUCCuGGUc -3' miRNA: 3'- -CGCCG--GGUCCag-CGG--CCAUAGG-CCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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