Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23045 | 3' | -62.4 | NC_005178.1 | + | 11876 | 0.66 | 0.303237 |
Target: 5'- cGCGGUcaCCAGGgcgGCCagGGUGUCCuGGUc -3' miRNA: 3'- -CGCCG--GGUCCag-CGG--CCAUAGG-CCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 11762 | 0.66 | 0.303237 |
Target: 5'- cGUGGCCCgagccauggGGGUucCGCCGcaggCCGGCc -3' miRNA: 3'- -CGCCGGG---------UCCA--GCGGCcauaGGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 10294 | 0.74 | 0.078124 |
Target: 5'- cCGGCCau-GUCGCggaCGGUAUCCGGCa -3' miRNA: 3'- cGCCGGgucCAGCG---GCCAUAGGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 9659 | 0.66 | 0.268145 |
Target: 5'- cGCGGCCgGuGGcuUCGCCGag--CCGGCc -3' miRNA: 3'- -CGCCGGgU-CC--AGCGGCcauaGGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 9443 | 0.68 | 0.213333 |
Target: 5'- cCGGCCUAgccgggacGGUCuGCCGGacguggugGUCCGGUa -3' miRNA: 3'- cGCCGGGU--------CCAG-CGGCCa-------UAGGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 9095 | 0.68 | 0.213333 |
Target: 5'- cGCGGCauugcuCCAGGUCgaaGCCGGcaa-CGGCg -3' miRNA: 3'- -CGCCG------GGUCCAG---CGGCCauagGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 5923 | 0.71 | 0.115282 |
Target: 5'- gGCGGCCgAGG--GCCGGUGUagCGGCc -3' miRNA: 3'- -CGCCGGgUCCagCGGCCAUAg-GCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 5880 | 0.75 | 0.064141 |
Target: 5'- gGCGGCCguugCGGGUCa--GGUAUCCGGCg -3' miRNA: 3'- -CGCCGG----GUCCAGcggCCAUAGGCCGa -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 5210 | 0.7 | 0.155448 |
Target: 5'- cCGGCgCCAGGUUGCgGGcGUCCuccagagacacuGGCUg -3' miRNA: 3'- cGCCG-GGUCCAGCGgCCaUAGG------------CCGA- -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 4028 | 0.71 | 0.128274 |
Target: 5'- aGCGGCCgGGccucGUCGUCGGUgacgcgcAUCgGGCUg -3' miRNA: 3'- -CGCCGGgUC----CAGCGGCCA-------UAGgCCGA- -5' |
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23045 | 3' | -62.4 | NC_005178.1 | + | 1516 | 0.69 | 0.181888 |
Target: 5'- gGCGGCguaguugcacucaCCAGGUauaGCgCGGg--CCGGCUg -3' miRNA: 3'- -CGCCG-------------GGUCCAg--CG-GCCauaGGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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