Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23045 | 5' | -54.9 | NC_005178.1 | + | 28281 | 0.68 | 0.52463 |
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Target: 5'- gUCAGUGGGGUgaCCGgCGCUGcgggagCUGCc -3' miRNA: 3'- -GGUCGUCCUAa-GGCgGCGACua----GACG- -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 36341 | 0.67 | 0.557648 |
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Target: 5'- aCAGCGGacGAUucgCCGgcaucaucaauCCGCUGAuUCUGCg -3' miRNA: 3'- gGUCGUC--CUAa--GGC-----------GGCGACU-AGACG- -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 13560 | 0.67 | 0.579988 |
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Target: 5'- gCGGCGGGucagCGUCGuCUGAUCcGCg -3' miRNA: 3'- gGUCGUCCuaagGCGGC-GACUAGaCG- -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 29540 | 0.67 | 0.591231 |
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Target: 5'- cCCAGCGGGAggagcgUCCGaUUGCUGuaGUCggacucguugGCa -3' miRNA: 3'- -GGUCGUCCUa-----AGGC-GGCGAC--UAGa---------CG- -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 25970 | 0.66 | 0.60251 |
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Target: 5'- gCCAgGCuGGugaccaCGUCGgUGAUCUGCg -3' miRNA: 3'- -GGU-CGuCCuaag--GCGGCgACUAGACG- -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 16436 | 0.66 | 0.62514 |
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Target: 5'- cCCAgggcGUAGGcg-CCGCCGCccgacucgcUGGUCUGg -3' miRNA: 3'- -GGU----CGUCCuaaGGCGGCG---------ACUAGACg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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