Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23045 | 5' | -54.9 | NC_005178.1 | + | 16698 | 0.69 | 0.43086 |
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Target: 5'- gCAGCGGGAUUCCcaGCUGCUc--CUGg -3' miRNA: 3'- gGUCGUCCUAAGG--CGGCGAcuaGACg -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 32970 | 0.7 | 0.374078 |
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Target: 5'- gCCGGCGaccacgccgcuGGAgaucacCCaGCCGCUGAugcUCUGCg -3' miRNA: 3'- -GGUCGU-----------CCUaa----GG-CGGCGACU---AGACG- -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 34395 | 0.71 | 0.322589 |
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Target: 5'- uCCcGCAGGAg-CCGgaUGCUGAUCUGUc -3' miRNA: 3'- -GGuCGUCCUaaGGCg-GCGACUAGACG- -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 24577 | 0.73 | 0.242365 |
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Target: 5'- aCCGGCGacGGuguugCCGUCGCUGAUCaaUGCc -3' miRNA: 3'- -GGUCGU--CCuaa--GGCGGCGACUAG--ACG- -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 24606 | 1.14 | 0.000289 |
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Target: 5'- uCCAGCAGGAUUCCGCCGCUGAUCUGCa -3' miRNA: 3'- -GGUCGUCCUAAGGCGGCGACUAGACG- -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 16436 | 0.66 | 0.62514 |
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Target: 5'- cCCAgggcGUAGGcg-CCGCCGCccgacucgcUGGUCUGg -3' miRNA: 3'- -GGU----CGUCCuaaGGCGGCG---------ACUAGACg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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