Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23045 | 5' | -54.9 | NC_005178.1 | + | 11767 | 0.73 | 0.276578 |
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Target: 5'- cCCgAGCcaugGGGGUUCCGCCGCaggccGGcCUGCa -3' miRNA: 3'- -GG-UCG----UCCUAAGGCGGCGa----CUaGACG- -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 8912 | 0.72 | 0.291307 |
|
Target: 5'- gCCAGCAGGAUcggcccggcuUCCGUgCGCUGGgggUGUg -3' miRNA: 3'- -GGUCGUCCUA----------AGGCG-GCGACUag-ACG- -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 5028 | 0.68 | 0.52463 |
|
Target: 5'- aCCAGguGGuccAUUUCGUCGaUGAUCaGCg -3' miRNA: 3'- -GGUCguCC---UAAGGCGGCgACUAGaCG- -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 4949 | 0.68 | 0.52463 |
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Target: 5'- gCCAGCGG----CCGcCCGCUGAUCa-- -3' miRNA: 3'- -GGUCGUCcuaaGGC-GGCGACUAGacg -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 4119 | 0.72 | 0.306643 |
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Target: 5'- gCCAGCAGGGUgugggUCUacgacggcgaggGCCGCUuccUCUGCa -3' miRNA: 3'- -GGUCGUCCUA-----AGG------------CGGCGAcu-AGACG- -5' |
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| 23045 | 5' | -54.9 | NC_005178.1 | + | 3665 | 0.7 | 0.41137 |
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Target: 5'- aCCAGagccuguGGAUUCgCGCCGCcaagGAaCUGCc -3' miRNA: 3'- -GGUCgu-----CCUAAG-GCGGCGa---CUaGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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