Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23049 | 5' | -58.2 | NC_005178.1 | + | 31500 | 0.66 | 0.409554 |
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Target: 5'- ----gGCCGGCCucgacuuccgcgacgGCGGUC-UGGCGa- -3' miRNA: 3'- cuuuaCGGCCGG---------------UGCCGGuACCGCag -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 34738 | 0.66 | 0.415274 |
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Target: 5'- gGAAcgGCUGGCCAggguucaGGCUAcgGGCG-Cg -3' miRNA: 3'- -CUUuaCGGCCGGUg------CCGGUa-CCGCaG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 4492 | 0.66 | 0.424917 |
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Target: 5'- -cGGUGgUGGCUugGGUgGUGGCugcgGUCg -3' miRNA: 3'- cuUUACgGCCGGugCCGgUACCG----CAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 3946 | 0.66 | 0.434692 |
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Target: 5'- --uGUGgCGGCgGCGGCC--GGUGUUc -3' miRNA: 3'- cuuUACgGCCGgUGCCGGuaCCGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 28771 | 0.66 | 0.434692 |
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Target: 5'- ---uUGCCGGCCcacauguCGGCCGccaucUGGUcgggGUCu -3' miRNA: 3'- cuuuACGGCCGGu------GCCGGU-----ACCG----CAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 7068 | 0.66 | 0.434692 |
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Target: 5'- uGAGUGcCCGGcCCugGGCgA-GGCGg- -3' miRNA: 3'- cUUUAC-GGCC-GGugCCGgUaCCGCag -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 6871 | 0.66 | 0.434692 |
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Target: 5'- cGAucUGUCGGCCugGGgCGa-GCGUCc -3' miRNA: 3'- -CUuuACGGCCGGugCCgGUacCGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 9656 | 0.66 | 0.438638 |
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Target: 5'- ---cUGCgCGGCCGguggcuucgccgagcCGGCCGaGGCGgUCa -3' miRNA: 3'- cuuuACG-GCCGGU---------------GCCGGUaCCGC-AG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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