Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23049 | 5' | -58.2 | NC_005178.1 | + | 28991 | 0.71 | 0.198663 |
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Target: 5'- --cGUGCCGGaCGCGGggAUGGUGUCg -3' miRNA: 3'- cuuUACGGCCgGUGCCggUACCGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 17126 | 0.7 | 0.22739 |
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Target: 5'- -cAAUGgCGGCCucCGGCCGcaUGGCGa- -3' miRNA: 3'- cuUUACgGCCGGu-GCCGGU--ACCGCag -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 18453 | 0.7 | 0.239832 |
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Target: 5'- -cGAUGCCGcGCaucaGCGGCag-GGUGUCg -3' miRNA: 3'- cuUUACGGC-CGg---UGCCGguaCCGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 3823 | 0.69 | 0.259556 |
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Target: 5'- -uGGUGCCgcccuuGGCgAUGGCCcgGcGCGUCa -3' miRNA: 3'- cuUUACGG------CCGgUGCCGGuaC-CGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 23511 | 0.69 | 0.27272 |
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Target: 5'- ----cGCCGGgCGCccuGGCCGUcgcccugGGCGUCg -3' miRNA: 3'- cuuuaCGGCCgGUG---CCGGUA-------CCGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 4718 | 0.69 | 0.280584 |
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Target: 5'- -cGAUGUCGGCCAugccauUGGCCAguugGGUGg- -3' miRNA: 3'- cuUUACGGCCGGU------GCCGGUa---CCGCag -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 3428 | 0.69 | 0.287887 |
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Target: 5'- gGAAggGCCGGCCGugcaucgggugcUGGaCCucGGCGUCg -3' miRNA: 3'- -CUUuaCGGCCGGU------------GCC-GGuaCCGCAG- -5' |
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| 23049 | 5' | -58.2 | NC_005178.1 | + | 25902 | 1.1 | 0.000224 |
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Target: 5'- cGAAAUGCCGGCCACGGCCAUGGCGUCa -3' miRNA: 3'- -CUUUACGGCCGGUGCCGGUACCGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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