Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23055 | 3' | -53.5 | NC_005178.1 | + | 20382 | 0.66 | 0.726132 |
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Target: 5'- cCGCCCuGAAGCc--GGCCcuggacGCCCAGGc -3' miRNA: 3'- -GUGGGcCUUCGuuaUUGGc-----UGGGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 23337 | 0.66 | 0.726132 |
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Target: 5'- cUACCUGGgcGaCGA-GAUCGGCaCCGGGg -3' miRNA: 3'- -GUGGGCCuuC-GUUaUUGGCUG-GGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 23124 | 0.66 | 0.726132 |
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Target: 5'- -uCCCGGAGGaaau-ACCG-CCCAGc -3' miRNA: 3'- guGGGCCUUCguuauUGGCuGGGUCc -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 24838 | 0.66 | 0.737051 |
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Target: 5'- gCACCUGGAggaacgccucGGCAAUGGCCuugagGGCCgCGuGGu -3' miRNA: 3'- -GUGGGCCU----------UCGUUAUUGG-----CUGG-GU-CC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 31998 | 0.66 | 0.737051 |
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Target: 5'- cCGCUCGGcucGGCccauGCCGAUCCAGc -3' miRNA: 3'- -GUGGGCCu--UCGuuauUGGCUGGGUCc -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 10296 | 0.66 | 0.737051 |
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Target: 5'- aGCUCGGcGGCAcguccuggcggAUAGCCGAgggCCGGGc -3' miRNA: 3'- gUGGGCCuUCGU-----------UAUUGGCUg--GGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 23175 | 0.66 | 0.737051 |
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Target: 5'- gCACCUGGuGAGCAcu-GCCGAgCCGu- -3' miRNA: 3'- -GUGGGCC-UUCGUuauUGGCUgGGUcc -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 5974 | 0.66 | 0.737051 |
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Target: 5'- aACCCGGAgucguuccggGGC----GCUGcCCCAGGc -3' miRNA: 3'- gUGGGCCU----------UCGuuauUGGCuGGGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 21075 | 0.66 | 0.737051 |
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Target: 5'- aGgCCGGuGGCGGUGcuGCCG-CCCAcGGc -3' miRNA: 3'- gUgGGCCuUCGUUAU--UGGCuGGGU-CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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