Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23055 | 3' | -53.5 | NC_005178.1 | + | 5228 | 0.7 | 0.460148 |
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Target: 5'- cCACuCCGG-GGCuguaGACCGGCgCCAGGu -3' miRNA: 3'- -GUG-GGCCuUCGuua-UUGGCUG-GGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 4400 | 0.7 | 0.470502 |
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Target: 5'- uGCUCGGggGUGAUcguGCCGAggCCGGGg -3' miRNA: 3'- gUGGGCCuuCGUUAu--UGGCUg-GGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 16797 | 0.69 | 0.510872 |
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Target: 5'- uCACCUGGGccagcgcAGCGAUGcgaggaccagggcGCCGGCUCAGu -3' miRNA: 3'- -GUGGGCCU-------UCGUUAU-------------UGGCUGGGUCc -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 8886 | 0.69 | 0.513038 |
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Target: 5'- aCACCCGGcacgcgccGGGCGAcuuucuuGCCGACCUGGu -3' miRNA: 3'- -GUGGGCC--------UUCGUUau-----UGGCUGGGUCc -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 34638 | 0.69 | 0.513038 |
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Target: 5'- uCGCCCGGccAGCuccccGGUGGCaCG-CCCAGGa -3' miRNA: 3'- -GUGGGCCu-UCG-----UUAUUG-GCuGGGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 17819 | 0.69 | 0.541515 |
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Target: 5'- aACCCGGcaucgGGGCGAaauuggaucuuccGACCGGCCCGGu -3' miRNA: 3'- gUGGGCC-----UUCGUUa------------UUGGCUGGGUCc -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 27155 | 0.68 | 0.579499 |
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Target: 5'- cCGCCUGGAucgcGGCAAUGcuCCGGUCCAGc -3' miRNA: 3'- -GUGGGCCU----UCGUUAUu-GGCUGGGUCc -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 20451 | 0.68 | 0.602115 |
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Target: 5'- uCGCCUGGgcGUccagGGCCGGCUuCAGGg -3' miRNA: 3'- -GUGGGCCuuCGuua-UUGGCUGG-GUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 30720 | 1.09 | 0.00096 |
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Target: 5'- uCACCCGGAAGCAAUAACCGACCCAGGc -3' miRNA: 3'- -GUGGGCCUUCGUUAUUGGCUGGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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