Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23106 | 5' | -56 | NC_005178.1 | + | 30438 | 0.7 | 0.299108 |
Target: 5'- ---cGGCGCCG---GCCGCUGGAggUa -3' miRNA: 3'- gauaCCGUGGCgagCGGCGACCUuuG- -5' |
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23106 | 5' | -56 | NC_005178.1 | + | 23288 | 0.71 | 0.284015 |
Target: 5'- --uUGGCAUCGUUCGCCGUaacgaUGGugccGACu -3' miRNA: 3'- gauACCGUGGCGAGCGGCG-----ACCu---UUG- -5' |
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23106 | 5' | -56 | NC_005178.1 | + | 22652 | 0.71 | 0.284015 |
Target: 5'- -gAUGGCGCUGgggUCGCgGCUGGAGc- -3' miRNA: 3'- gaUACCGUGGCg--AGCGgCGACCUUug -5' |
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23106 | 5' | -56 | NC_005178.1 | + | 31061 | 0.72 | 0.248929 |
Target: 5'- ---aGGCGUCGC-CGCCGCUGGGuuuuGCu -3' miRNA: 3'- gauaCCGUGGCGaGCGGCGACCUu---UG- -5' |
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23106 | 5' | -56 | NC_005178.1 | + | 11627 | 0.72 | 0.229645 |
Target: 5'- -aGUGGCAucCCGCUgGCCagGCUGGuGACc -3' miRNA: 3'- gaUACCGU--GGCGAgCGG--CGACCuUUG- -5' |
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23106 | 5' | -56 | NC_005178.1 | + | 4008 | 0.72 | 0.229645 |
Target: 5'- --cUGcGCACCGUcCGCCGCUGcGAACu -3' miRNA: 3'- gauAC-CGUGGCGaGCGGCGACcUUUG- -5' |
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23106 | 5' | -56 | NC_005178.1 | + | 12835 | 1.1 | 0.000361 |
Target: 5'- cCUAUGGCACCGCUCGCCGCUGGAAACa -3' miRNA: 3'- -GAUACCGUGGCGAGCGGCGACCUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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