Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23106 | 5' | -56 | NC_005178.1 | + | 25321 | 0.68 | 0.402497 |
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Target: 5'- gCUGUcGGC-CCGCUUgGCCaggcGCUGGAAAa -3' miRNA: 3'- -GAUA-CCGuGGCGAG-CGG----CGACCUUUg -5' |
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| 23106 | 5' | -56 | NC_005178.1 | + | 4635 | 0.7 | 0.322898 |
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Target: 5'- ---cGGCauaGCCgGCgaccaCGCCGCUGGAGAUc -3' miRNA: 3'- gauaCCG---UGG-CGa----GCGGCGACCUUUG- -5' |
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| 23106 | 5' | -56 | NC_005178.1 | + | 21186 | 0.7 | 0.310037 |
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Target: 5'- ---aGGCGCCGC-CGCCcgacucgcuggucugGCUGGuGAGCg -3' miRNA: 3'- gauaCCGUGGCGaGCGG---------------CGACC-UUUG- -5' |
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| 23106 | 5' | -56 | NC_005178.1 | + | 30438 | 0.7 | 0.299108 |
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Target: 5'- ---cGGCGCCG---GCCGCUGGAggUa -3' miRNA: 3'- gauaCCGUGGCgagCGGCGACCUuuG- -5' |
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| 23106 | 5' | -56 | NC_005178.1 | + | 22652 | 0.71 | 0.284015 |
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Target: 5'- -gAUGGCGCUGgggUCGCgGCUGGAGc- -3' miRNA: 3'- gaUACCGUGGCg--AGCGgCGACCUUug -5' |
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| 23106 | 5' | -56 | NC_005178.1 | + | 23288 | 0.71 | 0.284015 |
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Target: 5'- --uUGGCAUCGUUCGCCGUaacgaUGGugccGACu -3' miRNA: 3'- gauACCGUGGCGAGCGGCG-----ACCu---UUG- -5' |
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| 23106 | 5' | -56 | NC_005178.1 | + | 11627 | 0.72 | 0.229645 |
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Target: 5'- -aGUGGCAucCCGCUgGCCagGCUGGuGACc -3' miRNA: 3'- gaUACCGU--GGCGAgCGG--CGACCuUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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