Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23128 | 5' | -60.3 | NC_005178.1 | + | 21342 | 0.66 | 0.348767 |
Target: 5'- uGCCCGCUGCggcaUGGCCGaggCC-GGuCa -3' miRNA: 3'- -CGGGCGACG----GCCGGCaaaGGaCCuGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 6809 | 0.66 | 0.348767 |
Target: 5'- uGCCCuGCUGCCGauccGCCGccucauugCCUGG-Ca -3' miRNA: 3'- -CGGG-CGACGGC----CGGCaaa-----GGACCuGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 25336 | 0.66 | 0.324453 |
Target: 5'- gGCCaggCGCUggaaaagaGCCGcGCUGgugUCCUGGACc -3' miRNA: 3'- -CGG---GCGA--------CGGC-CGGCaa-AGGACCUGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 12945 | 0.66 | 0.324453 |
Target: 5'- cGCCaGgUGCUGGCCGg--CCUGGu-- -3' miRNA: 3'- -CGGgCgACGGCCGGCaaaGGACCuga -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 2978 | 0.67 | 0.312011 |
Target: 5'- cGCCCgagcgcggaugGCUGCCGaGCgugcgcagccuggagCGcUUCCUGGACc -3' miRNA: 3'- -CGGG-----------CGACGGC-CG---------------GCaAAGGACCUGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 31461 | 0.67 | 0.308957 |
Target: 5'- -aCCGCUGgCGGCCGgcUCgCUGaACa -3' miRNA: 3'- cgGGCGACgGCCGGCaaAG-GACcUGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 6089 | 0.67 | 0.301423 |
Target: 5'- uGUUCaGCgaGCCGGCCGccagcggUUCCUGGAg- -3' miRNA: 3'- -CGGG-CGa-CGGCCGGCa------AAGGACCUga -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 12308 | 0.67 | 0.286783 |
Target: 5'- uCgCGCUGCCuGCCGcUUUCCUGcuGGCg -3' miRNA: 3'- cGgGCGACGGcCGGC-AAAGGAC--CUGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 32617 | 0.67 | 0.286783 |
Target: 5'- cGgCCGCUGgCGGCCagUUCCUcGGCg -3' miRNA: 3'- -CgGGCGACgGCCGGcaAAGGAcCUGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 13017 | 0.67 | 0.284635 |
Target: 5'- aGCCCGCcgugaaggugcagcUGCUGGCCag--CCUGGcCg -3' miRNA: 3'- -CGGGCG--------------ACGGCCGGcaaaGGACCuGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 23258 | 0.68 | 0.272708 |
Target: 5'- aUCCGCUuGCCGgaGCCGaagaCCUGGACc -3' miRNA: 3'- cGGGCGA-CGGC--CGGCaaa-GGACCUGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 15280 | 0.68 | 0.272708 |
Target: 5'- cGCCCGCcGCCuGGCgGgccagCCUGG-Cg -3' miRNA: 3'- -CGGGCGaCGG-CCGgCaaa--GGACCuGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 14090 | 0.68 | 0.259193 |
Target: 5'- gGCCgGCUucgcgccgggcGCCcuGGCCGUcgcCCUGGGCg -3' miRNA: 3'- -CGGgCGA-----------CGG--CCGGCAaa-GGACCUGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 18847 | 0.68 | 0.259193 |
Target: 5'- cGUgCGCUuggcuugaGCUGGCCGUUUCUgaGGACc -3' miRNA: 3'- -CGgGCGA--------CGGCCGGCAAAGGa-CCUGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 6772 | 0.68 | 0.252643 |
Target: 5'- -aCCGCUGCCaGGCgGaugUCCUGGuucaGCUc -3' miRNA: 3'- cgGGCGACGG-CCGgCaa-AGGACC----UGA- -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 22158 | 0.68 | 0.24623 |
Target: 5'- cGCgCUGCUGCCGGUuaCGgcUUCUGGcaGCUg -3' miRNA: 3'- -CG-GGCGACGGCCG--GCaaAGGACC--UGA- -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 33928 | 0.69 | 0.227798 |
Target: 5'- cGgCCGgUGCCGGCU--UUCCgGGGCg -3' miRNA: 3'- -CgGGCgACGGCCGGcaAAGGaCCUGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 11908 | 0.69 | 0.216169 |
Target: 5'- gGCCUGCUGCCGGUCGaa--CUcGGCg -3' miRNA: 3'- -CGGGCGACGGCCGGCaaagGAcCUGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 25176 | 0.69 | 0.216169 |
Target: 5'- aGCCaagGCcGCCGGuaGUggCCUGGGCg -3' miRNA: 3'- -CGGg--CGaCGGCCggCAaaGGACCUGa -5' |
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23128 | 5' | -60.3 | NC_005178.1 | + | 34180 | 0.69 | 0.210547 |
Target: 5'- gGCCgGaaggGCCGGCCGUgcaucgggUgCUGGACc -3' miRNA: 3'- -CGGgCga--CGGCCGGCAa-------AgGACCUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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