Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23142 | 3' | -65.7 | NC_005178.1 | + | 31305 | 0.71 | 0.073053 |
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Target: 5'- cCAGCGcCGGGCuaugcGCCUGGCGCCgCg- -3' miRNA: 3'- uGUCGC-GCUCGu----CGGGCCGCGGgGag -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 11142 | 0.71 | 0.069069 |
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Target: 5'- uCAGCGCGGGC-GCCCuGGCcgccGCCCUg- -3' miRNA: 3'- uGUCGCGCUCGuCGGG-CCG----CGGGGag -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 14685 | 0.71 | 0.063483 |
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Target: 5'- gACGGCGCGAucaucuGCuGgCCGGaugggcaGCCCCUCu -3' miRNA: 3'- -UGUCGCGCU------CGuCgGGCCg------CGGGGAG- -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 20665 | 0.72 | 0.053591 |
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Target: 5'- -gGGCGcCGAGCAGCUCGGCcucGCUaCCUCc -3' miRNA: 3'- ugUCGC-GCUCGUCGGGCCG---CGG-GGAG- -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 16885 | 0.73 | 0.046506 |
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Target: 5'- gUAGCGaggcCGAGCuGCUCGGCGCCCUg- -3' miRNA: 3'- uGUCGC----GCUCGuCGGGCCGCGGGGag -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 27199 | 0.74 | 0.041386 |
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Target: 5'- cCAGCuCGGccagcGCAGCCCGGCgcgcuucGCCCCUCc -3' miRNA: 3'- uGUCGcGCU-----CGUCGGGCCG-------CGGGGAG- -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 18496 | 0.74 | 0.040338 |
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Target: 5'- uGCAGCGCGAGCggcgaaggGGCgCGGCGCgcaCCCg- -3' miRNA: 3'- -UGUCGCGCUCG--------UCGgGCCGCG---GGGag -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 24232 | 1.08 | 0.000056 |
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Target: 5'- gACAGCGCGAGCAGCCCGGCGCCCCUCg -3' miRNA: 3'- -UGUCGCGCUCGUCGGGCCGCGGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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