Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23155 | 3' | -59.4 | NC_005178.1 | + | 16720 | 0.71 | 0.190357 |
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Target: 5'- gCCgGCGCCcugguccucgcAUCgCUGCgCugGCCCAGGu -3' miRNA: 3'- -GGgCGCGG-----------UAGgGAUG-GugCGGGUUC- -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 28645 | 0.72 | 0.175622 |
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Target: 5'- gCCGgGCCGaUCCUG-CugGCCCAGGg -3' miRNA: 3'- gGGCgCGGUaGGGAUgGugCGGGUUC- -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 20870 | 0.72 | 0.166371 |
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Target: 5'- gCCUGCGCCGg-CCUGau-CGCCCAGGa -3' miRNA: 3'- -GGGCGCGGUagGGAUgguGCGGGUUC- -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 12759 | 0.74 | 0.126396 |
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Target: 5'- aCCCGCGaCG-CCCUgcGCCGCGCCUAc- -3' miRNA: 3'- -GGGCGCgGUaGGGA--UGGUGCGGGUuc -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 20428 | 0.74 | 0.126396 |
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Target: 5'- gCUGCGCgAaaCCCUcgGCCugGCCCAGGa -3' miRNA: 3'- gGGCGCGgUa-GGGA--UGGugCGGGUUC- -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 14097 | 0.74 | 0.122928 |
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Target: 5'- uUCGCGCCGggcgCCCUgGCCGuCGCCCuGGg -3' miRNA: 3'- gGGCGCGGUa---GGGA-UGGU-GCGGGuUC- -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 10787 | 0.75 | 0.106878 |
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Target: 5'- gCCGcCGCCAUCCUgGCCGaGCCCAGc -3' miRNA: 3'- gGGC-GCGGUAGGGaUGGUgCGGGUUc -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 2976 | 0.75 | 0.092811 |
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Target: 5'- gCCCG-GCCAgcUCCCcggUGgCACGCCCAGGa -3' miRNA: 3'- -GGGCgCGGU--AGGG---AUgGUGCGGGUUC- -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 28589 | 1.09 | 0.000269 |
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Target: 5'- uCCCGCGCCAUCCCUACCACGCCCAAGc -3' miRNA: 3'- -GGGCGCGGUAGGGAUGGUGCGGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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