Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23155 | 3' | -59.4 | NC_005178.1 | + | 28589 | 1.09 | 0.000269 |
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Target: 5'- uCCCGCGCCAUCCCUACCACGCCCAAGc -3' miRNA: 3'- -GGGCGCGGUAGGGAUGGUGCGGGUUC- -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 28645 | 0.72 | 0.175622 |
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Target: 5'- gCCGgGCCGaUCCUG-CugGCCCAGGg -3' miRNA: 3'- gGGCgCGGUaGGGAUgGugCGGGUUC- -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 28961 | 0.68 | 0.295461 |
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Target: 5'- gCCgUGCGCCAggugaCCCUG-CGCGCCCcuGg -3' miRNA: 3'- -GG-GCGCGGUa----GGGAUgGUGCGGGuuC- -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 29252 | 0.66 | 0.42147 |
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Target: 5'- gCCgGCGCCGgcuucaUCCUGCCACcuuGUCCGc- -3' miRNA: 3'- -GGgCGCGGUa-----GGGAUGGUG---CGGGUuc -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 31303 | 0.67 | 0.342082 |
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Target: 5'- aCCCaGCGCCGggcuaugcgCCUggcGCCGCGCCgGGa -3' miRNA: 3'- -GGG-CGCGGUa--------GGGa--UGGUGCGGgUUc -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 31588 | 0.66 | 0.393787 |
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Target: 5'- aCCGcCGCCggCCUggACCugGUCCGc- -3' miRNA: 3'- gGGC-GCGGuaGGGa-UGGugCGGGUuc -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 32267 | 0.66 | 0.412112 |
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Target: 5'- gCUCGCGCCggCCCUgaacgguggcgGCCuCGgCCAAc -3' miRNA: 3'- -GGGCGCGGuaGGGA-----------UGGuGCgGGUUc -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 34522 | 0.7 | 0.235034 |
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Target: 5'- aCCaggGCGCCAcgCUCgGCCugGUCCAGGa -3' miRNA: 3'- -GGg--CGCGGUa-GGGaUGGugCGGGUUC- -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 34714 | 0.67 | 0.333956 |
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Target: 5'- aCCCgGCGCggCAUCCCUgaaACCGuCGUUCGGGu -3' miRNA: 3'- -GGG-CGCG--GUAGGGA---UGGU-GCGGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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