Results 41 - 49 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23155 | 3' | -59.4 | NC_005178.1 | + | 16485 | 0.69 | 0.250058 |
Target: 5'- aUCCGCGCCAUUgCgguuguCCACguauuccucagcgcgGCCCAGGu -3' miRNA: 3'- -GGGCGCGGUAGgGau----GGUG---------------CGGGUUC- -5' |
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23155 | 3' | -59.4 | NC_005178.1 | + | 4493 | 0.69 | 0.250058 |
Target: 5'- aCCCGCaguugGCCA--CCUGCCGgaaugcccucggugcCGCCCAGGa -3' miRNA: 3'- -GGGCG-----CGGUagGGAUGGU---------------GCGGGUUC- -5' |
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23155 | 3' | -59.4 | NC_005178.1 | + | 34522 | 0.7 | 0.235034 |
Target: 5'- aCCaggGCGCCAcgCUCgGCCugGUCCAGGa -3' miRNA: 3'- -GGg--CGCGGUa-GGGaUGGugCGGGUUC- -5' |
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23155 | 3' | -59.4 | NC_005178.1 | + | 27221 | 0.7 | 0.217327 |
Target: 5'- -gCGCGCUucgcCCCU-CCGCGCCCGGc -3' miRNA: 3'- ggGCGCGGua--GGGAuGGUGCGGGUUc -5' |
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23155 | 3' | -59.4 | NC_005178.1 | + | 9425 | 0.7 | 0.217327 |
Target: 5'- -gCGcCGCCAUCggUGCCAcCGCCCAGGc -3' miRNA: 3'- ggGC-GCGGUAGggAUGGU-GCGGGUUC- -5' |
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23155 | 3' | -59.4 | NC_005178.1 | + | 28645 | 0.72 | 0.175622 |
Target: 5'- gCCGgGCCGaUCCUG-CugGCCCAGGg -3' miRNA: 3'- gGGCgCGGUaGGGAUgGugCGGGUUC- -5' |
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23155 | 3' | -59.4 | NC_005178.1 | + | 20870 | 0.72 | 0.166371 |
Target: 5'- gCCUGCGCCGg-CCUGau-CGCCCAGGa -3' miRNA: 3'- -GGGCGCGGUagGGAUgguGCGGGUUC- -5' |
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23155 | 3' | -59.4 | NC_005178.1 | + | 14097 | 0.74 | 0.122928 |
Target: 5'- uUCGCGCCGggcgCCCUgGCCGuCGCCCuGGg -3' miRNA: 3'- gGGCGCGGUa---GGGA-UGGU-GCGGGuUC- -5' |
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23155 | 3' | -59.4 | NC_005178.1 | + | 2976 | 0.75 | 0.092811 |
Target: 5'- gCCCG-GCCAgcUCCCcggUGgCACGCCCAGGa -3' miRNA: 3'- -GGGCgCGGU--AGGG---AUgGUGCGGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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