Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23155 | 3' | -59.4 | NC_005178.1 | + | 9019 | 0.67 | 0.333151 |
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Target: 5'- cUCCGCGCCGUUgccggcuucgaCCUggagcaaugccgcGCCGCGCUCGc- -3' miRNA: 3'- -GGGCGCGGUAG-----------GGA-------------UGGUGCGGGUuc -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 34714 | 0.67 | 0.333956 |
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Target: 5'- aCCCgGCGCggCAUCCCUgaaACCGuCGUUCGGGu -3' miRNA: 3'- -GGG-CGCG--GUAGGGA---UGGU-GCGGGUUC- -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 9380 | 0.67 | 0.342082 |
|
Target: 5'- aCCGCaGCaggaaAUCCaCUacACCACGgCCAAGa -3' miRNA: 3'- gGGCG-CGg----UAGG-GA--UGGUGCgGGUUC- -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 19365 | 0.67 | 0.342082 |
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Target: 5'- aCCCGCGCUA--CCUGCgCGggcUGCUCAAGa -3' miRNA: 3'- -GGGCGCGGUagGGAUG-GU---GCGGGUUC- -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 31303 | 0.67 | 0.342082 |
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Target: 5'- aCCCaGCGCCGggcuaugcgCCUggcGCCGCGCCgGGa -3' miRNA: 3'- -GGG-CGCGGUa--------GGGa--UGGUGCGGgUUc -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 15247 | 0.67 | 0.342082 |
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Target: 5'- gCUGCGCCAcuUCCCggccgcagACCACGacgauggcCCCGAc -3' miRNA: 3'- gGGCGCGGU--AGGGa-------UGGUGC--------GGGUUc -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 12150 | 0.67 | 0.350351 |
|
Target: 5'- aCCCGCGCCua-CC-GCgACGUCCuGGa -3' miRNA: 3'- -GGGCGCGGuagGGaUGgUGCGGGuUC- -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 1217 | 0.67 | 0.35876 |
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Target: 5'- gCCGCGCUGgaccugagCCCUGugaagcUCugGCCCGAa -3' miRNA: 3'- gGGCGCGGUa-------GGGAU------GGugCGGGUUc -5' |
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| 23155 | 3' | -59.4 | NC_005178.1 | + | 7175 | 0.67 | 0.365589 |
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Target: 5'- gCCGCGCCuGUCCacgcaacccgcaUACCGCcCCCAu- -3' miRNA: 3'- gGGCGCGG-UAGGg-----------AUGGUGcGGGUuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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