Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23164 | 3' | -56.1 | NC_005178.1 | + | 332 | 0.67 | 0.494016 |
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Target: 5'- gGCUGU-CAGG-UCCGG-CGCUGuGCc -3' miRNA: 3'- aCGACAuGUUCuGGGCCaGCGGCuCG- -5' |
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| 23164 | 3' | -56.1 | NC_005178.1 | + | 33710 | 0.67 | 0.494016 |
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Target: 5'- cUGCUGUGCGG---CCGGUCGuuGuacucGGCa -3' miRNA: 3'- -ACGACAUGUUcugGGCCAGCggC-----UCG- -5' |
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| 23164 | 3' | -56.1 | NC_005178.1 | + | 19107 | 0.67 | 0.504593 |
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Target: 5'- gGCUGgacgAUGAGGCUCGcucCGCCGaAGCg -3' miRNA: 3'- aCGACa---UGUUCUGGGCca-GCGGC-UCG- -5' |
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| 23164 | 3' | -56.1 | NC_005178.1 | + | 17610 | 0.66 | 0.5478 |
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Target: 5'- cGCUGUGCGucGCCgUGGUCugacgGUCGAGUc -3' miRNA: 3'- aCGACAUGUucUGG-GCCAG-----CGGCUCG- -5' |
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| 23164 | 3' | -56.1 | NC_005178.1 | + | 16606 | 0.66 | 0.558793 |
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Target: 5'- aGCUGggcgucCAGGugCCGGUCaaCUGGGUc -3' miRNA: 3'- aCGACau----GUUCugGGCCAGc-GGCUCG- -5' |
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| 23164 | 3' | -56.1 | NC_005178.1 | + | 28880 | 0.66 | 0.569847 |
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Target: 5'- cGCgaacCcAGACCUGGUCGCggcUGGGCa -3' miRNA: 3'- aCGacauGuUCUGGGCCAGCG---GCUCG- -5' |
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| 23164 | 3' | -56.1 | NC_005178.1 | + | 18057 | 0.66 | 0.569847 |
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Target: 5'- ---cGUaACA--GCCCGGUCGCucgCGAGCa -3' miRNA: 3'- acgaCA-UGUucUGGGCCAGCG---GCUCG- -5' |
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| 23164 | 3' | -56.1 | NC_005178.1 | + | 5342 | 0.66 | 0.569847 |
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Target: 5'- aGCUGUACAccGGugC-GGcC-CCGAGCc -3' miRNA: 3'- aCGACAUGU--UCugGgCCaGcGGCUCG- -5' |
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| 23164 | 3' | -56.1 | NC_005178.1 | + | 28713 | 0.66 | 0.569847 |
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Target: 5'- gGC-GU-CGGGgcucacACCCGGcacgCGCCGGGCg -3' miRNA: 3'- aCGaCAuGUUC------UGGGCCa---GCGGCUCG- -5' |
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| 23164 | 3' | -56.1 | NC_005178.1 | + | 32902 | 0.66 | 0.580953 |
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Target: 5'- gGCUGggugaucucCAGcGGCgUGGUCGCCG-GCa -3' miRNA: 3'- aCGACau-------GUU-CUGgGCCAGCGGCuCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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