Results 21 - 30 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23164 | 3' | -56.1 | NC_005178.1 | + | 10452 | 0.69 | 0.376392 |
Target: 5'- cGCUGguacggugGCGcGAUCUGGagaUCGUCGAGCg -3' miRNA: 3'- aCGACa-------UGUuCUGGGCC---AGCGGCUCG- -5' |
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23164 | 3' | -56.1 | NC_005178.1 | + | 21602 | 0.7 | 0.350072 |
Target: 5'- aUGCUGUuaucgcGCAAGcgcagcucguCCUGGUCGuCCGGGUu -3' miRNA: 3'- -ACGACA------UGUUCu---------GGGCCAGC-GGCUCG- -5' |
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23164 | 3' | -56.1 | NC_005178.1 | + | 17321 | 0.7 | 0.341594 |
Target: 5'- cGCcGgacgGCGGGGCCgaGGUCGCCcAGCu -3' miRNA: 3'- aCGaCa---UGUUCUGGg-CCAGCGGcUCG- -5' |
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23164 | 3' | -56.1 | NC_005178.1 | + | 6718 | 0.7 | 0.341594 |
Target: 5'- cUGCUGaGCcaggAGGugCCGccaugCGCCGAGCa -3' miRNA: 3'- -ACGACaUG----UUCugGGCca---GCGGCUCG- -5' |
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23164 | 3' | -56.1 | NC_005178.1 | + | 17883 | 0.71 | 0.293875 |
Target: 5'- ---cGUGCuccuGGACCCGGUaCGCC-AGCg -3' miRNA: 3'- acgaCAUGu---UCUGGGCCA-GCGGcUCG- -5' |
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23164 | 3' | -56.1 | NC_005178.1 | + | 7511 | 0.72 | 0.25821 |
Target: 5'- cGC-GUGaauGGCCCGG-CGCUGAGCu -3' miRNA: 3'- aCGaCAUguuCUGGGCCaGCGGCUCG- -5' |
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23164 | 3' | -56.1 | NC_005178.1 | + | 11292 | 0.73 | 0.203009 |
Target: 5'- aGCUGUuCGAGuccGCCCagguGGUgGCCGGGCu -3' miRNA: 3'- aCGACAuGUUC---UGGG----CCAgCGGCUCG- -5' |
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23164 | 3' | -56.1 | NC_005178.1 | + | 36209 | 0.74 | 0.197552 |
Target: 5'- aGCUGguagGCGGGAUggCCGacgCGCCGAGCg -3' miRNA: 3'- aCGACa---UGUUCUG--GGCca-GCGGCUCG- -5' |
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23164 | 3' | -56.1 | NC_005178.1 | + | 26462 | 0.75 | 0.153969 |
Target: 5'- gGCUGUuucGGAUaCGGUCGCCGAGUu -3' miRNA: 3'- aCGACAuguUCUGgGCCAGCGGCUCG- -5' |
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23164 | 3' | -56.1 | NC_005178.1 | + | 32617 | 1.12 | 0.0003 |
Target: 5'- cUGCUGUACAAGACCCGGUCGCCGAGCg -3' miRNA: 3'- -ACGACAUGUUCUGGGCCAGCGGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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