Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23168 | 3' | -56 | NC_005178.1 | + | 12191 | 0.66 | 0.537499 |
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Target: 5'- cGGaCAGCUcccgGCCAGCcuggcuGGCAUGGUCGc -3' miRNA: 3'- -UCaGUUGAug--CGGUCG------CCGUGCCAGU- -5' |
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| 23168 | 3' | -56 | NC_005178.1 | + | 34072 | 0.66 | 0.537499 |
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Target: 5'- cAGuUCAuCUG-GCUAGCGG-ACGGUCAg -3' miRNA: 3'- -UC-AGUuGAUgCGGUCGCCgUGCCAGU- -5' |
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| 23168 | 3' | -56 | NC_005178.1 | + | 2506 | 0.66 | 0.537499 |
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Target: 5'- -aUCAACgaacGCGCCAgagcugccgacGCGGU-CGGUCAg -3' miRNA: 3'- ucAGUUGa---UGCGGU-----------CGCCGuGCCAGU- -5' |
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| 23168 | 3' | -56 | NC_005178.1 | + | 32560 | 0.66 | 0.548512 |
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Target: 5'- -aUCAGCUcgACcugGCCAGCGGCGaccaggUGGUCc -3' miRNA: 3'- ucAGUUGA--UG---CGGUCGCCGU------GCCAGu -5' |
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| 23168 | 3' | -56 | NC_005178.1 | + | 34523 | 0.66 | 0.548512 |
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Target: 5'- cGGUC-GCUGCGUCgcaggGGCGGCGCucGUCu -3' miRNA: 3'- -UCAGuUGAUGCGG-----UCGCCGUGc-CAGu -5' |
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| 23168 | 3' | -56 | NC_005178.1 | + | 29058 | 0.66 | 0.548512 |
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Target: 5'- --aCAACcuuCGCCugaugGGCGGCACGGcguUCAa -3' miRNA: 3'- ucaGUUGau-GCGG-----UCGCCGUGCC---AGU- -5' |
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| 23168 | 3' | -56 | NC_005178.1 | + | 8988 | 0.66 | 0.559595 |
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Target: 5'- uGcCGGCgACGCCcccGGUGGCGCGG-CGg -3' miRNA: 3'- uCaGUUGaUGCGG---UCGCCGUGCCaGU- -5' |
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| 23168 | 3' | -56 | NC_005178.1 | + | 28531 | 0.66 | 0.57074 |
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Target: 5'- aGGUCGAa---GCCGGCaacGGCGCGGagUCGg -3' miRNA: 3'- -UCAGUUgaugCGGUCG---CCGUGCC--AGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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