Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23168 | 3' | -56 | NC_005178.1 | + | 33689 | 0.69 | 0.357968 |
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Target: 5'- gGGUCAACgaUGCGCgGcaagcugcuguGCGGC-CGGUCGu -3' miRNA: 3'- -UCAGUUG--AUGCGgU-----------CGCCGuGCCAGU- -5' |
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| 23168 | 3' | -56 | NC_005178.1 | + | 33817 | 0.67 | 0.494287 |
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Target: 5'- cGUCAGCUugaaggugGCCAGCuuGGCcugGCGGUCc -3' miRNA: 3'- uCAGUUGAug------CGGUCG--CCG---UGCCAGu -5' |
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| 23168 | 3' | -56 | NC_005178.1 | + | 34072 | 0.66 | 0.537499 |
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Target: 5'- cAGuUCAuCUG-GCUAGCGG-ACGGUCAg -3' miRNA: 3'- -UC-AGUuGAUgCGGUCGCCgUGCCAGU- -5' |
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| 23168 | 3' | -56 | NC_005178.1 | + | 34523 | 0.66 | 0.548512 |
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Target: 5'- cGGUC-GCUGCGUCgcaggGGCGGCGCucGUCu -3' miRNA: 3'- -UCAGuUGAUGCGG-----UCGCCGUGc-CAGu -5' |
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| 23168 | 3' | -56 | NC_005178.1 | + | 35112 | 0.69 | 0.384906 |
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Target: 5'- ---gAGCUGcCGCUGGCGGC-CGGUCc -3' miRNA: 3'- ucagUUGAU-GCGGUCGCCGuGCCAGu -5' |
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| 23168 | 3' | -56 | NC_005178.1 | + | 35146 | 0.67 | 0.50495 |
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Target: 5'- cGUUGACgaACGCCAGcCGGCugGaggacGUCAc -3' miRNA: 3'- uCAGUUGa-UGCGGUC-GCCGugC-----CAGU- -5' |
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| 23168 | 3' | -56 | NC_005178.1 | + | 35227 | 1.08 | 0.000557 |
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Target: 5'- cAGUCAACUACGCCAGCGGCACGGUCAc -3' miRNA: 3'- -UCAGUUGAUGCGGUCGCCGUGCCAGU- -5' |
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| 23168 | 3' | -56 | NC_005178.1 | + | 36015 | 0.67 | 0.473279 |
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Target: 5'- cAGgcgCGAC-AUGCgGGCGGCgaccucguaACGGUCAa -3' miRNA: 3'- -UCa--GUUGaUGCGgUCGCCG---------UGCCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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