Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23191 | 3' | -55.6 | NC_005259.1 | + | 15416 | 0.66 | 0.77528 |
Target: 5'- aCACCGUgAgguguuccuuuuUGCUCGuCGG-GGUCAgcaGGCg -3' miRNA: 3'- -GUGGCAgU------------ACGAGU-GCCaCCGGU---UCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 2766 | 0.66 | 0.76932 |
Target: 5'- aCGCgGUCGUGCagccaucgacgcagCGCGGcccGcGCCGGGCg -3' miRNA: 3'- -GUGgCAGUACGa-------------GUGCCa--C-CGGUUCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 60642 | 0.66 | 0.76532 |
Target: 5'- uGCCGgUAUcGCUCgGCGGcGGCCucGGCa -3' miRNA: 3'- gUGGCaGUA-CGAG-UGCCaCCGGu-UCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 45556 | 0.66 | 0.764316 |
Target: 5'- cCACCGcCAagcagauUGCUuucgCACGG-GGCCucGGCa -3' miRNA: 3'- -GUGGCaGU-------ACGA----GUGCCaCCGGu-UCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 40726 | 0.66 | 0.762305 |
Target: 5'- aACCGUCG-GCUCugccgauguaggccACGGUGccGCCcuGCg -3' miRNA: 3'- gUGGCAGUaCGAG--------------UGCCAC--CGGuuCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 12510 | 0.66 | 0.755227 |
Target: 5'- aACCGUC---CUCgACGGUGGCgaugauucccuCAGGCa -3' miRNA: 3'- gUGGCAGuacGAG-UGCCACCG-----------GUUCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 16405 | 0.66 | 0.745012 |
Target: 5'- uCGCCGagCAccaGCgccgUACGGUGGCCAacuacgucgAGCu -3' miRNA: 3'- -GUGGCa-GUa--CGa---GUGCCACCGGU---------UCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 50235 | 0.66 | 0.734686 |
Target: 5'- aCAUgGcC-UGCcacaUCGCGGUGGgCAAGCg -3' miRNA: 3'- -GUGgCaGuACG----AGUGCCACCgGUUCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 64388 | 0.66 | 0.72426 |
Target: 5'- aCGCUGUCggGC---CGGUGGCgCGAGUu -3' miRNA: 3'- -GUGGCAGuaCGaguGCCACCG-GUUCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 55813 | 0.66 | 0.72426 |
Target: 5'- aGCuCGcCAUGCUCuaccaagcacaGCGG-GGCCA-GCg -3' miRNA: 3'- gUG-GCaGUACGAG-----------UGCCaCCGGUuCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 10071 | 0.67 | 0.713744 |
Target: 5'- -gUCGUCG-GCgggCGCGGUGGC--AGCg -3' miRNA: 3'- guGGCAGUaCGa--GUGCCACCGguUCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 68562 | 0.67 | 0.713744 |
Target: 5'- gCACCGaUCuacgUCACGGUGG-CAGGCc -3' miRNA: 3'- -GUGGC-AGuacgAGUGCCACCgGUUCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 33360 | 0.67 | 0.712688 |
Target: 5'- cCGCCGcCGcUGC-CGCuggcgagGGUGGCCAGcGCg -3' miRNA: 3'- -GUGGCaGU-ACGaGUG-------CCACCGGUU-CG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 52519 | 0.67 | 0.692491 |
Target: 5'- uCGCCGUCAaggGCgcacccgCGCGcaUGGCCGAGa -3' miRNA: 3'- -GUGGCAGUa--CGa------GUGCc-ACCGGUUCg -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 9147 | 0.67 | 0.681774 |
Target: 5'- aCACCGagggaUCAUGCUgGCucguuGUGGCCGaaaccgggGGCg -3' miRNA: 3'- -GUGGC-----AGUACGAgUGc----CACCGGU--------UCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 54772 | 0.67 | 0.671011 |
Target: 5'- gCGCgCGUCGUGCgUAUcG-GGCCGGGCa -3' miRNA: 3'- -GUG-GCAGUACGaGUGcCaCCGGUUCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 46296 | 0.67 | 0.671011 |
Target: 5'- uCGCCGUCAccgGCUCGauGagcuacGCCGAGCg -3' miRNA: 3'- -GUGGCAGUa--CGAGUgcCac----CGGUUCG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 68223 | 0.67 | 0.660213 |
Target: 5'- gCGCCGagGcgGCUgcccucggUugGGUGGCCAAGg -3' miRNA: 3'- -GUGGCagUa-CGA--------GugCCACCGGUUCg -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 13960 | 0.68 | 0.649389 |
Target: 5'- uGCCGagGUGCUUgccuCGGUGGCagucuGCg -3' miRNA: 3'- gUGGCagUACGAGu---GCCACCGguu--CG- -5' |
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23191 | 3' | -55.6 | NC_005259.1 | + | 16754 | 0.68 | 0.616864 |
Target: 5'- cUACCGcCcgGCcccucUCAgGGgGGCCGGGCa -3' miRNA: 3'- -GUGGCaGuaCG-----AGUgCCaCCGGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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