Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23191 | 5' | -52.2 | NC_005259.1 | + | 2041 | 0.66 | 0.902074 |
Target: 5'- cGAGCUguuucuugCGCGCCUcGCGGugAAUcGCGu -3' miRNA: 3'- -CUCGA--------GCGUGGA-CGCUugUUGuUGCu -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 22423 | 0.66 | 0.902074 |
Target: 5'- cGGCgucgacCGCGCCgcccgagGCGAGCAGCGcgucguugGCGGu -3' miRNA: 3'- cUCGa-----GCGUGGa------CGCUUGUUGU--------UGCU- -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 24770 | 0.66 | 0.895042 |
Target: 5'- cGAGCUCGCccgccgcgccGCCcGCGcGCGAgaaguCGGCGAc -3' miRNA: 3'- -CUCGAGCG----------UGGaCGCuUGUU-----GUUGCU- -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 65431 | 0.66 | 0.895042 |
Target: 5'- cGAGCUCGcCGCCUacaaGGACAuCGACa- -3' miRNA: 3'- -CUCGAGC-GUGGAcg--CUUGUuGUUGcu -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 16466 | 0.66 | 0.894324 |
Target: 5'- uGGGC-CGCGCCccaagauuggaugUGCGAGCccGACAugGu -3' miRNA: 3'- -CUCGaGCGUGG-------------ACGCUUG--UUGUugCu -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 8767 | 0.66 | 0.887737 |
Target: 5'- uGAGCUCGCuggcacGCUUGCGuGCGGguGCa- -3' miRNA: 3'- -CUCGAGCG------UGGACGCuUGUUguUGcu -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 66379 | 0.66 | 0.887737 |
Target: 5'- uGGCacuacCGCACCUcGcCGAACAAgGGCGGu -3' miRNA: 3'- cUCGa----GCGUGGA-C-GCUUGUUgUUGCU- -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 65910 | 0.66 | 0.880166 |
Target: 5'- aGAGC-CGCGCa-GCGAugAGCgGGCGGc -3' miRNA: 3'- -CUCGaGCGUGgaCGCUugUUG-UUGCU- -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 67811 | 0.67 | 0.872332 |
Target: 5'- uGAGC-CGCucACCUGCuacGACuGCAACGGc -3' miRNA: 3'- -CUCGaGCG--UGGACGc--UUGuUGUUGCU- -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 46115 | 0.67 | 0.864243 |
Target: 5'- -cGgUCGCGCgCUGCGucuguACGACGGCa- -3' miRNA: 3'- cuCgAGCGUG-GACGCu----UGUUGUUGcu -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 29257 | 0.67 | 0.847329 |
Target: 5'- uGGGC-CGgAUgUGCGAcagcgGCAGCAGCGGc -3' miRNA: 3'- -CUCGaGCgUGgACGCU-----UGUUGUUGCU- -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 58564 | 0.67 | 0.847329 |
Target: 5'- cGAGCUCGaCAUCgaGUGGcuCGACGGCGGu -3' miRNA: 3'- -CUCGAGC-GUGGa-CGCUu-GUUGUUGCU- -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 42497 | 0.67 | 0.83852 |
Target: 5'- cGAGCUCGcCGCCcgGCGAGaagagcGCggUGAg -3' miRNA: 3'- -CUCGAGC-GUGGa-CGCUUgu----UGuuGCU- -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 14117 | 0.67 | 0.83852 |
Target: 5'- cAGCagGcCACC-GCGAACggUAGCGAa -3' miRNA: 3'- cUCGagC-GUGGaCGCUUGuuGUUGCU- -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 43016 | 0.68 | 0.801159 |
Target: 5'- cGGGCa-GCGCCgagGUGAACAacggaaguaGCAGCGGg -3' miRNA: 3'- -CUCGagCGUGGa--CGCUUGU---------UGUUGCU- -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 37831 | 0.68 | 0.791338 |
Target: 5'- cGAGCUCGC-CCU-UGAGCcaguCGGCGAg -3' miRNA: 3'- -CUCGAGCGuGGAcGCUUGuu--GUUGCU- -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 25099 | 0.68 | 0.791338 |
Target: 5'- -uGCcCGCGCCgGCugaucgauGGGCAGCGACGAc -3' miRNA: 3'- cuCGaGCGUGGaCG--------CUUGUUGUUGCU- -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 60804 | 0.69 | 0.760901 |
Target: 5'- -cGUUuaCGCGCCcgaUGuCGGGCAACAGCGAg -3' miRNA: 3'- cuCGA--GCGUGG---AC-GCUUGUUGUUGCU- -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 27242 | 0.69 | 0.729246 |
Target: 5'- cGAcCUCGCAgCCUugcGCGAACAACAcCGGc -3' miRNA: 3'- -CUcGAGCGU-GGA---CGCUUGUUGUuGCU- -5' |
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23191 | 5' | -52.2 | NC_005259.1 | + | 51211 | 0.7 | 0.708714 |
Target: 5'- cGAGCgugCGCACCgcgauagcgaggccgGUGAGCu-CAGCGAg -3' miRNA: 3'- -CUCGa--GCGUGGa--------------CGCUUGuuGUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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