Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23199 | 5' | -57.4 | NC_005259.1 | + | 26826 | 0.69 | 0.485161 |
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Target: 5'- gGCGGuGGUGCCGGgGCGGUcuGCUcgGGCAg -3' miRNA: 3'- -CGCUcUUACGGCUgUGCCG--UGG--CCGU- -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 37185 | 0.69 | 0.486152 |
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Target: 5'- cGCGAGGAcgagcugcggcccuUGCCGcCACcgccgccgccgccggGGCGCUGGCc -3' miRNA: 3'- -CGCUCUU--------------ACGGCuGUG---------------CCGUGGCCGu -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 43884 | 0.69 | 0.495115 |
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Target: 5'- aGCGuuGccGCCGACAUugcccGCGCCGGCu -3' miRNA: 3'- -CGCucUuaCGGCUGUGc----CGUGGCCGu -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 8222 | 0.69 | 0.505161 |
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Target: 5'- aGCGAGcgugcgguGGUGCCGACACccuGCcuuACCGGUc -3' miRNA: 3'- -CGCUC--------UUACGGCUGUGc--CG---UGGCCGu -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 27997 | 0.68 | 0.515295 |
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Target: 5'- gGUGcGggUGCCGGgGCGGgguCGCCGGgGu -3' miRNA: 3'- -CGCuCuuACGGCUgUGCC---GUGGCCgU- -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 57446 | 0.68 | 0.522436 |
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Target: 5'- gGCGAGGuugGUG-CGACGCugcggcaggccgguGGCACCGGaCAc -3' miRNA: 3'- -CGCUCU---UACgGCUGUG--------------CCGUGGCC-GU- -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 67218 | 0.68 | 0.525509 |
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Target: 5'- gGCGGGGucgAUGCCGAcCACGGCGgUGuaGg -3' miRNA: 3'- -CGCUCU---UACGGCU-GUGCCGUgGCcgU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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