Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23199 | 5' | -57.4 | NC_005259.1 | + | 59291 | 0.68 | 0.525509 |
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Target: 5'- uGUGcAGAccuacgcUGUCGugGCGGCAgCGGCGa -3' miRNA: 3'- -CGC-UCUu------ACGGCugUGCCGUgGCCGU- -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 59402 | 0.68 | 0.525509 |
|
Target: 5'- cGCGAGcGUGaCCucgcGCAUGGCGUCGGCGg -3' miRNA: 3'- -CGCUCuUAC-GGc---UGUGCCGUGGCCGU- -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 61553 | 1.1 | 0.00071 |
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Target: 5'- gGCGAGAAUGCCGACACGGCACCGGCAg -3' miRNA: 3'- -CGCUCUUACGGCUGUGCCGUGGCCGU- -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 62720 | 0.66 | 0.683846 |
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Target: 5'- gGCGGGGGaucucGCuCGAC-CGG-ACCGGCGg -3' miRNA: 3'- -CGCUCUUa----CG-GCUGuGCCgUGGCCGU- -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 63659 | 0.73 | 0.303166 |
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Target: 5'- gGCcGGGAcGCCgGACACcucgGGCACCGGCGc -3' miRNA: 3'- -CGcUCUUaCGG-CUGUG----CCGUGGCCGU- -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 67218 | 0.68 | 0.525509 |
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Target: 5'- gGCGGGGucgAUGCCGAcCACGGCGgUGuaGg -3' miRNA: 3'- -CGCUCU---UACGGCU-GUGCCGUgGCcgU- -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 67956 | 0.71 | 0.391555 |
|
Target: 5'- uGCGGGuuGUGCUGuaGCGCGcGCAgCGGCGu -3' miRNA: 3'- -CGCUCu-UACGGC--UGUGC-CGUgGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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