Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23199 | 5' | -57.4 | NC_005259.1 | + | 35703 | 0.66 | 0.672212 |
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Target: 5'- gGCGAGGGgucGCUGACGaGGUAuccgcccgacgacCCGGCGc -3' miRNA: 3'- -CGCUCUUa--CGGCUGUgCCGU-------------GGCCGU- -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 62720 | 0.66 | 0.683846 |
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Target: 5'- gGCGGGGGaucucGCuCGAC-CGG-ACCGGCGg -3' miRNA: 3'- -CGCUCUUa----CG-GCUGuGCCgUGGCCGU- -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 6696 | 0.66 | 0.683846 |
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Target: 5'- -aGAGug-GCgCGACACGaucaACCGGCAg -3' miRNA: 3'- cgCUCuuaCG-GCUGUGCcg--UGGCCGU- -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 44463 | 0.66 | 0.694373 |
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Target: 5'- cCGGGGAUGCCGcCGCccuGGC-CgGGCc -3' miRNA: 3'- cGCUCUUACGGCuGUG---CCGuGgCCGu -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 47009 | 0.66 | 0.694373 |
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Target: 5'- cGCGAGGAaGCCGAcCACGaccgccuccaGCagGCCGGgGa -3' miRNA: 3'- -CGCUCUUaCGGCU-GUGC----------CG--UGGCCgU- -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 49351 | 0.66 | 0.694373 |
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Target: 5'- gGCGAGAgGUGCCG-CGCGaugaagagGCCGGUg -3' miRNA: 3'- -CGCUCU-UACGGCuGUGCcg------UGGCCGu -5' |
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| 23199 | 5' | -57.4 | NC_005259.1 | + | 17757 | 0.66 | 0.694373 |
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Target: 5'- cGUGAGGccGCCG-CGCGccgggccgagcGCACCGaGCGu -3' miRNA: 3'- -CGCUCUuaCGGCuGUGC-----------CGUGGC-CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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