Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23205 | 3' | -59.1 | NC_005259.1 | + | 59146 | 0.66 | 0.605947 |
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Target: 5'- uCGGCaGCGGUgcCGGGGAgCUGCUcaGGGCg -3' miRNA: 3'- -GCCGcCGCUA--GUCUCUaGGUGG--CCCG- -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 5006 | 0.66 | 0.605947 |
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Target: 5'- aCGGCaaGGUGGUC-GAGAUCgAUCcGGCc -3' miRNA: 3'- -GCCG--CCGCUAGuCUCUAGgUGGcCCG- -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 67528 | 0.66 | 0.612218 |
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Target: 5'- uCGGCGGCaAUCAccuugcccgcGAGAUCCucaggaugccaaCGGGUu -3' miRNA: 3'- -GCCGCCGcUAGU----------CUCUAGGug----------GCCCG- -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 65185 | 0.66 | 0.616403 |
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Target: 5'- aGGCgcuGGuCGAUCAGA--UCCGgUGGGUa -3' miRNA: 3'- gCCG---CC-GCUAGUCUcuAGGUgGCCCG- -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 23258 | 0.66 | 0.616403 |
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Target: 5'- gGGCGGUGGcCAccauGAGcaCCaagACCGGGCg -3' miRNA: 3'- gCCGCCGCUaGU----CUCuaGG---UGGCCCG- -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 60176 | 0.66 | 0.616403 |
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Target: 5'- gGGCGGCGAaCGccugcucGA-CCgACCGGGCc -3' miRNA: 3'- gCCGCCGCUaGUcu-----CUaGG-UGGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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