Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23205 | 3' | -59.1 | NC_005259.1 | + | 1976 | 0.72 | 0.300813 |
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Target: 5'- cCGGCGcuacacgaGCGGUCGGAGuacaacuacgcggCCAUCGGGUg -3' miRNA: 3'- -GCCGC--------CGCUAGUCUCua-----------GGUGGCCCG- -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 8779 | 0.72 | 0.284094 |
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Target: 5'- gCGGCGGUGAugagcUCGGcccgcuuggcgaGGAUCUucucgcGCCGGGCc -3' miRNA: 3'- -GCCGCCGCU-----AGUC------------UCUAGG------UGGCCCG- -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 41502 | 0.73 | 0.251638 |
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Target: 5'- gCGGUGGCGggCAGAccgugGAUCUGCaCGGGg -3' miRNA: 3'- -GCCGCCGCuaGUCU-----CUAGGUG-GCCCg -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 16542 | 0.75 | 0.16748 |
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Target: 5'- gGGUGcgcccgaGCGAcCAGAGAUCCcgcccACCGGGCa -3' miRNA: 3'- gCCGC-------CGCUaGUCUCUAGG-----UGGCCCG- -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 26824 | 0.76 | 0.159407 |
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Target: 5'- cCGGCGGUGGUgcCGGGGcgGUCUGCuCGGGCa -3' miRNA: 3'- -GCCGCCGCUA--GUCUC--UAGGUG-GCCCG- -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 58409 | 1.12 | 0.000404 |
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Target: 5'- cCGGCGGCGAUCAGAGAUCCACCGGGCa -3' miRNA: 3'- -GCCGCCGCUAGUCUCUAGGUGGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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