Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23205 | 3' | -59.1 | NC_005259.1 | + | 5342 | 0.69 | 0.435809 |
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Target: 5'- uGGUGGUGAgauucgCAuGAGccgaccuAUCgCGCCGGGCa -3' miRNA: 3'- gCCGCCGCUa-----GU-CUC-------UAG-GUGGCCCG- -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 62534 | 0.69 | 0.418549 |
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Target: 5'- uCGGCcucGaCGGUCcgAGAGAUagccUCACCGGGCa -3' miRNA: 3'- -GCCGc--C-GCUAG--UCUCUA----GGUGGCCCG- -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 8599 | 0.7 | 0.389582 |
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Target: 5'- cCGGCGGCcugaguugcuucauGGUCaccGGAGAUCCggcgagaauGgCGGGCa -3' miRNA: 3'- -GCCGCCG--------------CUAG---UCUCUAGG---------UgGCCCG- -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 57224 | 0.7 | 0.375181 |
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Target: 5'- cCGGUugucGG-GGUCGGAGAUCCacgucguuGCCGGGa -3' miRNA: 3'- -GCCG----CCgCUAGUCUCUAGG--------UGGCCCg -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 19217 | 0.7 | 0.371845 |
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Target: 5'- gGGCGGCGAcgugggacaccgagUCGGcGAUgaucgauggccgcCUGCCGGGCc -3' miRNA: 3'- gCCGCCGCU--------------AGUCuCUA-------------GGUGGCCCG- -5' |
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| 23205 | 3' | -59.1 | NC_005259.1 | + | 58409 | 1.12 | 0.000404 |
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Target: 5'- cCGGCGGCGAUCAGAGAUCCACCGGGCa -3' miRNA: 3'- -GCCGCCGCUAGUCUCUAGGUGGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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