Results 21 - 40 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23205 | 3' | -59.1 | NC_005259.1 | + | 65185 | 0.66 | 0.616403 |
Target: 5'- aGGCgcuGGuCGAUCAGA--UCCGgUGGGUa -3' miRNA: 3'- gCCG---CC-GCUAGUCUcuAGGUgGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 23258 | 0.66 | 0.616403 |
Target: 5'- gGGCGGUGGcCAccauGAGcaCCaagACCGGGCg -3' miRNA: 3'- gCCGCCGCUaGU----CUCuaGG---UGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 60176 | 0.66 | 0.616403 |
Target: 5'- gGGCGGCGAaCGccugcucGA-CCgACCGGGCc -3' miRNA: 3'- gCCGCCGCUaGUcu-----CUaGG-UGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 26695 | 0.7 | 0.361959 |
Target: 5'- aGGCGGgGAacUCGGGGuugauuuccucgaCCAUCGGGCc -3' miRNA: 3'- gCCGCCgCU--AGUCUCua-----------GGUGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 26824 | 0.76 | 0.159407 |
Target: 5'- cCGGCGGUGGUgcCGGGGcgGUCUGCuCGGGCa -3' miRNA: 3'- -GCCGCCGCUA--GUCUC--UAGGUG-GCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 16542 | 0.75 | 0.16748 |
Target: 5'- gGGUGcgcccgaGCGAcCAGAGAUCCcgcccACCGGGCa -3' miRNA: 3'- gCCGC-------CGCUaGUCUCUAGG-----UGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 41502 | 0.73 | 0.251638 |
Target: 5'- gCGGUGGCGggCAGAccgugGAUCUGCaCGGGg -3' miRNA: 3'- -GCCGCCGCuaGUCU-----CUAGGUG-GCCCg -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 8779 | 0.72 | 0.284094 |
Target: 5'- gCGGCGGUGAugagcUCGGcccgcuuggcgaGGAUCUucucgcGCCGGGCc -3' miRNA: 3'- -GCCGCCGCU-----AGUC------------UCUAGG------UGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 1976 | 0.72 | 0.300813 |
Target: 5'- cCGGCGcuacacgaGCGGUCGGAGuacaacuacgcggCCAUCGGGUg -3' miRNA: 3'- -GCCGC--------CGCUAGUCUCua-----------GGUGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 8655 | 0.71 | 0.312375 |
Target: 5'- aGGCuGUGGUCGGGcugCCACgCGGGCa -3' miRNA: 3'- gCCGcCGCUAGUCUcuaGGUG-GCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 61029 | 0.71 | 0.312375 |
Target: 5'- uCGGUGGCGG-C-GAGGUCgACCgacGGGCg -3' miRNA: 3'- -GCCGCCGCUaGuCUCUAGgUGG---CCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 8370 | 0.71 | 0.319026 |
Target: 5'- uCGGCGGCGGccucggcaUCGGcGAgagCCucggcgaGCCGGGCc -3' miRNA: 3'- -GCCGCCGCU--------AGUCuCUa--GG-------UGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 32877 | 0.71 | 0.327298 |
Target: 5'- gCGcGCGGUGAUC---GAUUCACCGGGg -3' miRNA: 3'- -GC-CGCCGCUAGucuCUAGGUGGCCCg -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 36117 | 0.71 | 0.334955 |
Target: 5'- uGGUGaGCGuaagCAGuuGggCCGCCGGGCu -3' miRNA: 3'- gCCGC-CGCua--GUCu-CuaGGUGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 54510 | 0.7 | 0.350659 |
Target: 5'- uGGCGGCGAUguGGGGU--GgCGGGUc -3' miRNA: 3'- gCCGCCGCUAguCUCUAggUgGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 58409 | 1.12 | 0.000404 |
Target: 5'- cCGGCGGCGAUCAGAGAUCCACCGGGCa -3' miRNA: 3'- -GCCGCCGCUAGUCUCUAGGUGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 19217 | 0.7 | 0.371845 |
Target: 5'- gGGCGGCGAcgugggacaccgagUCGGcGAUgaucgauggccgcCUGCCGGGCc -3' miRNA: 3'- gCCGCCGCU--------------AGUCuCUA-------------GGUGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 57224 | 0.7 | 0.375181 |
Target: 5'- cCGGUugucGG-GGUCGGAGAUCCacgucguuGCCGGGa -3' miRNA: 3'- -GCCG----CCgCUAGUCUCUAGG--------UGGCCCg -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 8599 | 0.7 | 0.389582 |
Target: 5'- cCGGCGGCcugaguugcuucauGGUCaccGGAGAUCCggcgagaauGgCGGGCa -3' miRNA: 3'- -GCCGCCG--------------CUAG---UCUCUAGG---------UgGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 62534 | 0.69 | 0.418549 |
Target: 5'- uCGGCcucGaCGGUCcgAGAGAUagccUCACCGGGCa -3' miRNA: 3'- -GCCGc--C-GCUAG--UCUCUA----GGUGGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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