Results 21 - 40 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23205 | 3' | -59.1 | NC_005259.1 | + | 34072 | 0.68 | 0.481163 |
Target: 5'- aCGGCGGCGGUguccacacgUAGGcccuguaccacaucGAgaacgugUCGCCGGGCg -3' miRNA: 3'- -GCCGCCGCUA---------GUCU--------------CUa------GGUGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 60344 | 0.68 | 0.481163 |
Target: 5'- aGGCGGCGAUCAGGucgaggcccugcccGAaaUCCcgccaaucGCCGGacaGCg -3' miRNA: 3'- gCCGCCGCUAGUCU--------------CU--AGG--------UGGCC---CG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 26376 | 0.68 | 0.474402 |
Target: 5'- uCGGUGGCGAgccuguugcUCGcGAu-UCCgacGCCGGGCg -3' miRNA: 3'- -GCCGCCGCU---------AGU-CUcuAGG---UGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 66480 | 0.68 | 0.474402 |
Target: 5'- uGGCGGUGGUCucgaucauGGGAuuuccuuccugUCgGCgGGGCg -3' miRNA: 3'- gCCGCCGCUAGu-------CUCU-----------AGgUGgCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 51645 | 0.68 | 0.464828 |
Target: 5'- gCGGUaGGCGAaCGGGuugCCGCgCGGGCg -3' miRNA: 3'- -GCCG-CCGCUaGUCUcuaGGUG-GCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 23312 | 0.68 | 0.455356 |
Target: 5'- cCGGC-GCGGUCGGugucgccgagccGGAUUaCAUCGGGCu -3' miRNA: 3'- -GCCGcCGCUAGUC------------UCUAG-GUGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 7039 | 0.68 | 0.455356 |
Target: 5'- cCGGCGGgGuuuuUCGuugcgcGAGAUCCugucgaCGGGCg -3' miRNA: 3'- -GCCGCCgCu---AGU------CUCUAGGug----GCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 18705 | 0.68 | 0.49384 |
Target: 5'- aCGGCGGUcugaccucGAUCAucGggCCGCCcGGCa -3' miRNA: 3'- -GCCGCCG--------CUAGUcuCuaGGUGGcCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 5342 | 0.69 | 0.435809 |
Target: 5'- uGGUGGUGAgauucgCAuGAGccgaccuAUCgCGCCGGGCa -3' miRNA: 3'- gCCGCCGCUa-----GU-CUC-------UAG-GUGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 62534 | 0.69 | 0.418549 |
Target: 5'- uCGGCcucGaCGGUCcgAGAGAUagccUCACCGGGCa -3' miRNA: 3'- -GCCGc--C-GCUAG--UCUCUA----GGUGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 54510 | 0.7 | 0.350659 |
Target: 5'- uGGCGGCGAUguGGGGU--GgCGGGUc -3' miRNA: 3'- gCCGCCGCUAguCUCUAggUgGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 26695 | 0.7 | 0.361959 |
Target: 5'- aGGCGGgGAacUCGGGGuugauuuccucgaCCAUCGGGCc -3' miRNA: 3'- gCCGCCgCU--AGUCUCua-----------GGUGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 19217 | 0.7 | 0.371845 |
Target: 5'- gGGCGGCGAcgugggacaccgagUCGGcGAUgaucgauggccgcCUGCCGGGCc -3' miRNA: 3'- gCCGCCGCU--------------AGUCuCUA-------------GGUGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 57224 | 0.7 | 0.375181 |
Target: 5'- cCGGUugucGG-GGUCGGAGAUCCacgucguuGCCGGGa -3' miRNA: 3'- -GCCG----CCgCUAGUCUCUAGG--------UGGCCCg -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 8599 | 0.7 | 0.389582 |
Target: 5'- cCGGCGGCcugaguugcuucauGGUCaccGGAGAUCCggcgagaauGgCGGGCa -3' miRNA: 3'- -GCCGCCG--------------CUAG---UCUCUAGG---------UgGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 36117 | 0.71 | 0.334955 |
Target: 5'- uGGUGaGCGuaagCAGuuGggCCGCCGGGCu -3' miRNA: 3'- gCCGC-CGCua--GUCu-CuaGGUGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 32877 | 0.71 | 0.327298 |
Target: 5'- gCGcGCGGUGAUC---GAUUCACCGGGg -3' miRNA: 3'- -GC-CGCCGCUAGucuCUAGGUGGCCCg -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 8370 | 0.71 | 0.319026 |
Target: 5'- uCGGCGGCGGccucggcaUCGGcGAgagCCucggcgaGCCGGGCc -3' miRNA: 3'- -GCCGCCGCU--------AGUCuCUa--GG-------UGGCCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 61029 | 0.71 | 0.312375 |
Target: 5'- uCGGUGGCGG-C-GAGGUCgACCgacGGGCg -3' miRNA: 3'- -GCCGCCGCUaGuCUCUAGgUGG---CCCG- -5' |
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23205 | 3' | -59.1 | NC_005259.1 | + | 8655 | 0.71 | 0.312375 |
Target: 5'- aGGCuGUGGUCGGGcugCCACgCGGGCa -3' miRNA: 3'- gCCGcCGCUAGUCUcuaGGUG-GCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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