Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23207 | 5' | -53.1 | NC_005259.1 | + | 29908 | 0.71 | 0.642398 |
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Target: 5'- -aGGGGugGcUGcccGAGACCaUCGACGCg -3' miRNA: 3'- agCCUCugCaAC---UUCUGG-AGCUGCGg -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 39554 | 0.7 | 0.689802 |
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Target: 5'- ----cGACGUUGAGccggucguccggcccGACCUCGAUGCUg -3' miRNA: 3'- agccuCUGCAACUU---------------CUGGAGCUGCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 13212 | 0.7 | 0.717381 |
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Target: 5'- gCGGGGccaGCGgu--GGGCCUCGACcgGCCa -3' miRNA: 3'- aGCCUC---UGCaacuUCUGGAGCUG--CGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 28138 | 0.7 | 0.717381 |
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Target: 5'- aCGuAGGCGaUGAGcugcccGGCCUCGACGCg -3' miRNA: 3'- aGCcUCUGCaACUU------CUGGAGCUGCGg -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 67240 | 0.69 | 0.73823 |
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Target: 5'- gCGGuguAGGgGUUGAgcgGGGCCUCGGgcaGCCg -3' miRNA: 3'- aGCC---UCUgCAACU---UCUGGAGCUg--CGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 14305 | 0.69 | 0.758665 |
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Target: 5'- gUCGGuG-CGUaugGccGcCCUCGACGCCg -3' miRNA: 3'- -AGCCuCuGCAa--CuuCuGGAGCUGCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 23739 | 0.68 | 0.797945 |
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Target: 5'- aCGuGGGACGgc---GACCcCGACGCCc -3' miRNA: 3'- aGC-CUCUGCaacuuCUGGaGCUGCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 61076 | 0.66 | 0.910622 |
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Target: 5'- aUCGGcauAGGCGUcGucGGCCUCGGgcuuguugaGCCa -3' miRNA: 3'- -AGCC---UCUGCAaCuuCUGGAGCUg--------CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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