Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23207 | 5' | -53.1 | NC_005259.1 | + | 53499 | 0.68 | 0.816621 |
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Target: 5'- uUCGGAu-CGgUGAAcaacGugCUCGugGCCa -3' miRNA: 3'- -AGCCUcuGCaACUU----CugGAGCugCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 57791 | 1.14 | 0.001326 |
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Target: 5'- gUCGGAGACGUUGAAGACCUCGACGCCg -3' miRNA: 3'- -AGCCUCUGCAACUUCUGGAGCUGCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 58162 | 0.66 | 0.897389 |
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Target: 5'- cUCGucGGCGcacGGGAUCUCGACGCa -3' miRNA: 3'- -AGCcuCUGCaacUUCUGGAGCUGCGg -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 61076 | 0.66 | 0.910622 |
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Target: 5'- aUCGGcauAGGCGUcGucGGCCUCGGgcuuguugaGCCa -3' miRNA: 3'- -AGCC---UCUGCAaCuuCUGGAGCUg--------CGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 63233 | 0.72 | 0.599124 |
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Target: 5'- aUCGGGGuCGcUGGucAGACC-CGACGCg -3' miRNA: 3'- -AGCCUCuGCaACU--UCUGGaGCUGCGg -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 67240 | 0.69 | 0.73823 |
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Target: 5'- gCGGuguAGGgGUUGAgcgGGGCCUCGGgcaGCCg -3' miRNA: 3'- aGCC---UCUgCAACU---UCUGGAGCUg--CGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 68403 | 0.68 | 0.825682 |
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Target: 5'- cUGGAccaccaACGU---AGACCUUGACGCCu -3' miRNA: 3'- aGCCUc-----UGCAacuUCUGGAGCUGCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 68554 | 0.77 | 0.314743 |
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Target: 5'- aUCGGGGugGUUGAgccaaccgagguGGACCUCGuauccgGCgGCCa -3' miRNA: 3'- -AGCCUCugCAACU------------UCUGGAGC------UG-CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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