Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23207 | 5' | -53.1 | NC_005259.1 | + | 24389 | 0.68 | 0.811094 |
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Target: 5'- cCGGAGuACGUcGAAcgcaccgugcucaauGACCUCGGCGa- -3' miRNA: 3'- aGCCUC-UGCAaCUU---------------CUGGAGCUGCgg -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 23739 | 0.68 | 0.797945 |
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Target: 5'- aCGuGGGACGgc---GACCcCGACGCCc -3' miRNA: 3'- aGC-CUCUGCaacuuCUGGaGCUGCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 20468 | 0.67 | 0.851648 |
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Target: 5'- cUCGGAuGAcCGccGucGACCUCGACaCCg -3' miRNA: 3'- -AGCCU-CU-GCaaCuuCUGGAGCUGcGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 18237 | 0.72 | 0.599124 |
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Target: 5'- cCGGAGACGgagauuucgagcUgcccgccgcgccUGAAccugagacGACCUCGACGCCc -3' miRNA: 3'- aGCCUCUGC------------A------------ACUU--------CUGGAGCUGCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 14305 | 0.69 | 0.758665 |
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Target: 5'- gUCGGuG-CGUaugGccGcCCUCGACGCCg -3' miRNA: 3'- -AGCCuCuGCAa--CuuCuGGAGCUGCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 13212 | 0.7 | 0.717381 |
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Target: 5'- gCGGGGccaGCGgu--GGGCCUCGACcgGCCa -3' miRNA: 3'- aGCCUC---UGCaacuUCUGGAGCUG--CGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 5598 | 0.67 | 0.874848 |
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Target: 5'- gCGGAGugcUGUgugaguuUGAAGACCUCauaGCCg -3' miRNA: 3'- aGCCUCu--GCA-------ACUUCUGGAGcugCGG- -5' |
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| 23207 | 5' | -53.1 | NC_005259.1 | + | 1546 | 0.68 | 0.816621 |
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Target: 5'- aUCGGGGccGCGUUcGGGGCggUGACGCUg -3' miRNA: 3'- -AGCCUC--UGCAAcUUCUGgaGCUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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